Glycine response in isolated dorsal cochlear nucleus of C57BL/6J mouse

Seiji Kakehata; Takashi Nakagawa; Tomonori Takasaka; Norio Akaike

doi:10.1016/0006-8993(92)90794-A

Glycine response in isolated dorsal cochlear nucleus of C57BL/6J mouse

Seiji Kakehata, Takashi Nakagawa, Tomonori Takasaka, Norio Akaike

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Pharmacological properties of glycine (Gly)-induced Cl^- current (I_Cl) in the dorsal cochlear nucleus (DCN) neurons acutely dissociated from C57BL/6J mouse were investigated in the whole-cell configuration of the patch-clamp technique. Gly-induced I_Cl increased in a sigmoidal manner with higher Gly concentrations. Strychnine blocked the Gly response competitively at low and non-competitively at high concentrations. Both glutamate (Glu) and N-methyl-d-aspartate (NMDA) responses were augmented by adding 10^-6 M Gly, at which concentration Gly did not induce any I_Cl. This facilitation was not affected by strychnine. Our results clearly show the existence of strychnine-sensitive and -insensitive glycine receptors in the DCN neurons.

Original language	English
Pages (from-to)	21-25
Number of pages	5
Journal	Brain Research
Volume	574
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(92)90794-A
Publication status	Published - Mar 6 1992
Externally published	Yes

All Science Journal Classification (ASJC) codes

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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title = "Glycine response in isolated dorsal cochlear nucleus of C57BL/6J mouse",

abstract = "Pharmacological properties of glycine (Gly)-induced Cl- current (ICl) in the dorsal cochlear nucleus (DCN) neurons acutely dissociated from C57BL/6J mouse were investigated in the whole-cell configuration of the patch-clamp technique. Gly-induced ICl increased in a sigmoidal manner with higher Gly concentrations. Strychnine blocked the Gly response competitively at low and non-competitively at high concentrations. Both glutamate (Glu) and N-methyl-d-aspartate (NMDA) responses were augmented by adding 10-6 M Gly, at which concentration Gly did not induce any ICl. This facilitation was not affected by strychnine. Our results clearly show the existence of strychnine-sensitive and -insensitive glycine receptors in the DCN neurons.",

author = "Seiji Kakehata and Takashi Nakagawa and Tomonori Takasaka and Norio Akaike",

year = "1992",

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doi = "10.1016/0006-8993(92)90794-A",

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T1 - Glycine response in isolated dorsal cochlear nucleus of C57BL/6J mouse

AU - Kakehata, Seiji

AU - Nakagawa, Takashi

AU - Takasaka, Tomonori

AU - Akaike, Norio

PY - 1992/3/6

Y1 - 1992/3/6

N2 - Pharmacological properties of glycine (Gly)-induced Cl- current (ICl) in the dorsal cochlear nucleus (DCN) neurons acutely dissociated from C57BL/6J mouse were investigated in the whole-cell configuration of the patch-clamp technique. Gly-induced ICl increased in a sigmoidal manner with higher Gly concentrations. Strychnine blocked the Gly response competitively at low and non-competitively at high concentrations. Both glutamate (Glu) and N-methyl-d-aspartate (NMDA) responses were augmented by adding 10-6 M Gly, at which concentration Gly did not induce any ICl. This facilitation was not affected by strychnine. Our results clearly show the existence of strychnine-sensitive and -insensitive glycine receptors in the DCN neurons.

AB - Pharmacological properties of glycine (Gly)-induced Cl- current (ICl) in the dorsal cochlear nucleus (DCN) neurons acutely dissociated from C57BL/6J mouse were investigated in the whole-cell configuration of the patch-clamp technique. Gly-induced ICl increased in a sigmoidal manner with higher Gly concentrations. Strychnine blocked the Gly response competitively at low and non-competitively at high concentrations. Both glutamate (Glu) and N-methyl-d-aspartate (NMDA) responses were augmented by adding 10-6 M Gly, at which concentration Gly did not induce any ICl. This facilitation was not affected by strychnine. Our results clearly show the existence of strychnine-sensitive and -insensitive glycine receptors in the DCN neurons.

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Glycine response in isolated dorsal cochlear nucleus of C57BL/6J mouse

Abstract

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