Glycine response in isolated dorsal cochlear nucleus of C57BL/6J mouse

Seiji Kakehata, Takashi Nakagawa, Tomonori Takasaka, Norio Akaike

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Pharmacological properties of glycine (Gly)-induced Cl- current (ICl) in the dorsal cochlear nucleus (DCN) neurons acutely dissociated from C57BL/6J mouse were investigated in the whole-cell configuration of the patch-clamp technique. Gly-induced ICl increased in a sigmoidal manner with higher Gly concentrations. Strychnine blocked the Gly response competitively at low and non-competitively at high concentrations. Both glutamate (Glu) and N-methyl-d-aspartate (NMDA) responses were augmented by adding 10-6 M Gly, at which concentration Gly did not induce any ICl. This facilitation was not affected by strychnine. Our results clearly show the existence of strychnine-sensitive and -insensitive glycine receptors in the DCN neurons.

Original languageEnglish
Pages (from-to)21-25
Number of pages5
JournalBrain Research
Volume574
Issue number1-2
DOIs
Publication statusPublished - Mar 6 1992
Externally publishedYes

Fingerprint

Cochlear Nucleus
Inbred C57BL Mouse
Glycine
Strychnine
Neurons
Patch-Clamp Techniques
Aspartic Acid
Glutamic Acid
Pharmacology

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Glycine response in isolated dorsal cochlear nucleus of C57BL/6J mouse. / Kakehata, Seiji; Nakagawa, Takashi; Takasaka, Tomonori; Akaike, Norio.

In: Brain Research, Vol. 574, No. 1-2, 06.03.1992, p. 21-25.

Research output: Contribution to journalArticle

Kakehata, Seiji ; Nakagawa, Takashi ; Takasaka, Tomonori ; Akaike, Norio. / Glycine response in isolated dorsal cochlear nucleus of C57BL/6J mouse. In: Brain Research. 1992 ; Vol. 574, No. 1-2. pp. 21-25.
@article{050b10c3faab481aa4ef8a79bbcdb296,
title = "Glycine response in isolated dorsal cochlear nucleus of C57BL/6J mouse",
abstract = "Pharmacological properties of glycine (Gly)-induced Cl- current (ICl) in the dorsal cochlear nucleus (DCN) neurons acutely dissociated from C57BL/6J mouse were investigated in the whole-cell configuration of the patch-clamp technique. Gly-induced ICl increased in a sigmoidal manner with higher Gly concentrations. Strychnine blocked the Gly response competitively at low and non-competitively at high concentrations. Both glutamate (Glu) and N-methyl-d-aspartate (NMDA) responses were augmented by adding 10-6 M Gly, at which concentration Gly did not induce any ICl. This facilitation was not affected by strychnine. Our results clearly show the existence of strychnine-sensitive and -insensitive glycine receptors in the DCN neurons.",
author = "Seiji Kakehata and Takashi Nakagawa and Tomonori Takasaka and Norio Akaike",
year = "1992",
month = "3",
day = "6",
doi = "10.1016/0006-8993(92)90794-A",
language = "English",
volume = "574",
pages = "21--25",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Glycine response in isolated dorsal cochlear nucleus of C57BL/6J mouse

AU - Kakehata, Seiji

AU - Nakagawa, Takashi

AU - Takasaka, Tomonori

AU - Akaike, Norio

PY - 1992/3/6

Y1 - 1992/3/6

N2 - Pharmacological properties of glycine (Gly)-induced Cl- current (ICl) in the dorsal cochlear nucleus (DCN) neurons acutely dissociated from C57BL/6J mouse were investigated in the whole-cell configuration of the patch-clamp technique. Gly-induced ICl increased in a sigmoidal manner with higher Gly concentrations. Strychnine blocked the Gly response competitively at low and non-competitively at high concentrations. Both glutamate (Glu) and N-methyl-d-aspartate (NMDA) responses were augmented by adding 10-6 M Gly, at which concentration Gly did not induce any ICl. This facilitation was not affected by strychnine. Our results clearly show the existence of strychnine-sensitive and -insensitive glycine receptors in the DCN neurons.

AB - Pharmacological properties of glycine (Gly)-induced Cl- current (ICl) in the dorsal cochlear nucleus (DCN) neurons acutely dissociated from C57BL/6J mouse were investigated in the whole-cell configuration of the patch-clamp technique. Gly-induced ICl increased in a sigmoidal manner with higher Gly concentrations. Strychnine blocked the Gly response competitively at low and non-competitively at high concentrations. Both glutamate (Glu) and N-methyl-d-aspartate (NMDA) responses were augmented by adding 10-6 M Gly, at which concentration Gly did not induce any ICl. This facilitation was not affected by strychnine. Our results clearly show the existence of strychnine-sensitive and -insensitive glycine receptors in the DCN neurons.

UR - http://www.scopus.com/inward/record.url?scp=0026604309&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026604309&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(92)90794-A

DO - 10.1016/0006-8993(92)90794-A

M3 - Article

VL - 574

SP - 21

EP - 25

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -