Glycopolymers Mimicking GM1 Gangliosides

Cooperativity of Galactose and Neuraminic Acid for Cholera Toxin Recognition

Yuhei Terada, Yu Hoshino, Yoshiko Miura

Research output: Contribution to journalArticle

Abstract

Glycopolymers mimicking GM1 gangliosides were synthesized by incorporating multiple types of carbohydrates into the polymer backbone. The glycopolymers were immobilized onto gold surfaces, and the interactions with the cholera toxin B subunit (CTB) were analyzed using surface plasmon resonance imaging. The glycopolymer containing both galactose and neuraminic acid showed enhanced recognition of CTB. The interaction was enhanced mainly because of an improvement in the dissociation process by the binding of the neuraminic acid group in the GM1 binding pocket. This cooperativity of galactose and neuraminic acid was achieved by incorporation into the same flexible polymer backbone, and the importance of the close placement of galactose and neuraminic acid groups was revealed. These results will be valuable in medical fields and also for the development of biofunctional materials.

Original languageEnglish
Pages (from-to)1021-1027
Number of pages7
JournalChemistry - An Asian Journal
Volume14
Issue number7
DOIs
Publication statusPublished - Apr 1 2019

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Neuraminic Acids
G(M1) Ganglioside
Cholera Toxin
Galactose
Polymers
Surface Plasmon Resonance
Surface plasmon resonance
Gold
Carbohydrates
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry

Cite this

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abstract = "Glycopolymers mimicking GM1 gangliosides were synthesized by incorporating multiple types of carbohydrates into the polymer backbone. The glycopolymers were immobilized onto gold surfaces, and the interactions with the cholera toxin B subunit (CTB) were analyzed using surface plasmon resonance imaging. The glycopolymer containing both galactose and neuraminic acid showed enhanced recognition of CTB. The interaction was enhanced mainly because of an improvement in the dissociation process by the binding of the neuraminic acid group in the GM1 binding pocket. This cooperativity of galactose and neuraminic acid was achieved by incorporation into the same flexible polymer backbone, and the importance of the close placement of galactose and neuraminic acid groups was revealed. These results will be valuable in medical fields and also for the development of biofunctional materials.",
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AU - Hoshino, Yu

AU - Miura, Yoshiko

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