Glycosphingolipids regulate ameloblastin expression in dental epithelial cells

Y. Kamasaki, T. Nakamura, Keigo Yoshizaki, T. Iwamoto, A. Yamada, E. Fukumoto, Y. Maruya, K. Iwabuchi, K. Furukawa, T. Fujiwara, Satoshi Fukumoto

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Neurotrophin 4 (NT-4) and its receptors regulate the differentiation of ameloblasts in tooth development. Gangliosides, sialic acids that contain glycosphingolipids (GSLs), are involved in a variety of membrane-associated cell physiological functions such as ligand-receptor signal transmission. However, the expression patterns and functions of GSLs during tooth development remain unclear. In this study, we identified strong expressions of GM3 and LacCer in dental epithelium, which give rise to differentiation into enamel-secreting ameloblasts. Exogenous GM3 and LacCer in dental epithelial cells induced the expression of ameloblastin (Ambn), while it was also interesting that GM3 synergistically exerted enhancement of NT-4-mediated Ambn expression. In addition, consistently exogenous GM3 and LacCer in dental epithelial cells induced distinct activation of extracellular signal-regulated kinase 1/2 (ERK1/2), an event upstream of the expression of Ambn. Furthermore, depletion of GSLs from dental epithelial cells by D-threo-1-phenyl-2-decanoylamino-3- morpholino-1-propanol (D-PDMP) inhibited Ambn expression as well as phosphorylation of ERK1/2. In contrast, exogenous addition of GM3 or LacCer rescued the phosphorylation of ERK1/2 repressed by pre-treatment with D-PDMP. Taken together, these results suggest that GM3 and LacCer are essential for NT-4-mediated Ambn expression, and contribute to dental epithelial cell differentiation into ameloblasts.

Original languageEnglish
Pages (from-to)78-83
Number of pages6
JournalJournal of Dental Research
Volume91
Issue number1
DOIs
Publication statusPublished - Jan 1 2012
Externally publishedYes

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Glycosphingolipids
Tooth
Epithelial Cells
Ameloblasts
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Phosphorylation
Sialic Acids
Gangliosides
Dental Enamel
Cell Differentiation
Epithelium
Cell Membrane
Ligands

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Glycosphingolipids regulate ameloblastin expression in dental epithelial cells. / Kamasaki, Y.; Nakamura, T.; Yoshizaki, Keigo; Iwamoto, T.; Yamada, A.; Fukumoto, E.; Maruya, Y.; Iwabuchi, K.; Furukawa, K.; Fujiwara, T.; Fukumoto, Satoshi.

In: Journal of Dental Research, Vol. 91, No. 1, 01.01.2012, p. 78-83.

Research output: Contribution to journalArticle

Kamasaki, Y, Nakamura, T, Yoshizaki, K, Iwamoto, T, Yamada, A, Fukumoto, E, Maruya, Y, Iwabuchi, K, Furukawa, K, Fujiwara, T & Fukumoto, S 2012, 'Glycosphingolipids regulate ameloblastin expression in dental epithelial cells', Journal of Dental Research, vol. 91, no. 1, pp. 78-83. https://doi.org/10.1177/0022034511424408
Kamasaki, Y. ; Nakamura, T. ; Yoshizaki, Keigo ; Iwamoto, T. ; Yamada, A. ; Fukumoto, E. ; Maruya, Y. ; Iwabuchi, K. ; Furukawa, K. ; Fujiwara, T. ; Fukumoto, Satoshi. / Glycosphingolipids regulate ameloblastin expression in dental epithelial cells. In: Journal of Dental Research. 2012 ; Vol. 91, No. 1. pp. 78-83.
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