Glycoxidized low-density lipoprotein enhances monocyte chemoattractant protein-1 mRNA expression in human umbilical vein endothelial cells

Relation to lysophosphatidylcholine contents and inhibition by nitric oxide donor

Kazuo Sonoki, Mototaka Yoshinari, Masanori Iwase, Kenzo Iino, Kojiro Ichikawa, Shigehiro Ohdo, Shun Higuchi, Mitsuo Iida

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Low-density lipoprotein (LDL) may undergo more glycation or oxidation in patients with diabetes mellitus than in nondiabetic subjects. We investigated whether glycoxidized LDL (goLDL) induces monocyte chemoattractant protein-1 (MCP-1) mRNA expression through activation of nuclear factor-kappaB (NFκB), and determined the effect of nitric oxide (NO) on MCP-1 mRNA expression in human umbilical vein endothelial cells (HUVEC). Oxidized (oxLDL) or goLDL enhanced MCP-1 mRNA expression in HUVEC, and preincubation with NOR3, a NO donor, abrogated such stimulation. goLDL increased NFκB-DNA binding activity in HUVEC and this effect was also suppressed by NOR3. We measured lysophosphatidylcholine (lyso-PC) contents in modified LDL using electrospray ionization liquid chromatography/mass spectrometry (LC/MS) to identify its molecular species. MCP-1 mRNA expression and NFκB activation correlated significantly with palmitoyl- and stearoyl-lyso-PC contents in LDL. Our results suggest that LDL modified by glycation and oxidation may contribute to the development of accelerated atherosclerosis in the presence of diabetes, a process that may be prevented by increased vascular NO availability.

Original languageEnglish
Pages (from-to)1135-1142
Number of pages8
JournalMetabolism: Clinical and Experimental
Volume51
Issue number9
DOIs
Publication statusPublished - Jan 1 2002

Fingerprint

Lysophosphatidylcholines
Nitric Oxide Donors
Chemokine CCL2
Human Umbilical Vein Endothelial Cells
LDL Lipoproteins
Messenger RNA
Nitric Oxide
Liquid Chromatography
Blood Vessels
Mass Spectrometry
Atherosclerosis
Diabetes Mellitus
DNA
oxidized low density lipoprotein

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Glycoxidized low-density lipoprotein enhances monocyte chemoattractant protein-1 mRNA expression in human umbilical vein endothelial cells : Relation to lysophosphatidylcholine contents and inhibition by nitric oxide donor. / Sonoki, Kazuo; Yoshinari, Mototaka; Iwase, Masanori; Iino, Kenzo; Ichikawa, Kojiro; Ohdo, Shigehiro; Higuchi, Shun; Iida, Mitsuo.

In: Metabolism: Clinical and Experimental, Vol. 51, No. 9, 01.01.2002, p. 1135-1142.

Research output: Contribution to journalArticle

@article{cd85b628c671462a92adcbde19f43cb7,
title = "Glycoxidized low-density lipoprotein enhances monocyte chemoattractant protein-1 mRNA expression in human umbilical vein endothelial cells: Relation to lysophosphatidylcholine contents and inhibition by nitric oxide donor",
abstract = "Low-density lipoprotein (LDL) may undergo more glycation or oxidation in patients with diabetes mellitus than in nondiabetic subjects. We investigated whether glycoxidized LDL (goLDL) induces monocyte chemoattractant protein-1 (MCP-1) mRNA expression through activation of nuclear factor-kappaB (NFκB), and determined the effect of nitric oxide (NO) on MCP-1 mRNA expression in human umbilical vein endothelial cells (HUVEC). Oxidized (oxLDL) or goLDL enhanced MCP-1 mRNA expression in HUVEC, and preincubation with NOR3, a NO donor, abrogated such stimulation. goLDL increased NFκB-DNA binding activity in HUVEC and this effect was also suppressed by NOR3. We measured lysophosphatidylcholine (lyso-PC) contents in modified LDL using electrospray ionization liquid chromatography/mass spectrometry (LC/MS) to identify its molecular species. MCP-1 mRNA expression and NFκB activation correlated significantly with palmitoyl- and stearoyl-lyso-PC contents in LDL. Our results suggest that LDL modified by glycation and oxidation may contribute to the development of accelerated atherosclerosis in the presence of diabetes, a process that may be prevented by increased vascular NO availability.",
author = "Kazuo Sonoki and Mototaka Yoshinari and Masanori Iwase and Kenzo Iino and Kojiro Ichikawa and Shigehiro Ohdo and Shun Higuchi and Mitsuo Iida",
year = "2002",
month = "1",
day = "1",
doi = "10.1053/meta.2002.34703",
language = "English",
volume = "51",
pages = "1135--1142",
journal = "Metabolism: Clinical and Experimental",
issn = "0026-0495",
publisher = "W.B. Saunders Ltd",
number = "9",

}

TY - JOUR

T1 - Glycoxidized low-density lipoprotein enhances monocyte chemoattractant protein-1 mRNA expression in human umbilical vein endothelial cells

T2 - Relation to lysophosphatidylcholine contents and inhibition by nitric oxide donor

AU - Sonoki, Kazuo

AU - Yoshinari, Mototaka

AU - Iwase, Masanori

AU - Iino, Kenzo

AU - Ichikawa, Kojiro

AU - Ohdo, Shigehiro

AU - Higuchi, Shun

AU - Iida, Mitsuo

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Low-density lipoprotein (LDL) may undergo more glycation or oxidation in patients with diabetes mellitus than in nondiabetic subjects. We investigated whether glycoxidized LDL (goLDL) induces monocyte chemoattractant protein-1 (MCP-1) mRNA expression through activation of nuclear factor-kappaB (NFκB), and determined the effect of nitric oxide (NO) on MCP-1 mRNA expression in human umbilical vein endothelial cells (HUVEC). Oxidized (oxLDL) or goLDL enhanced MCP-1 mRNA expression in HUVEC, and preincubation with NOR3, a NO donor, abrogated such stimulation. goLDL increased NFκB-DNA binding activity in HUVEC and this effect was also suppressed by NOR3. We measured lysophosphatidylcholine (lyso-PC) contents in modified LDL using electrospray ionization liquid chromatography/mass spectrometry (LC/MS) to identify its molecular species. MCP-1 mRNA expression and NFκB activation correlated significantly with palmitoyl- and stearoyl-lyso-PC contents in LDL. Our results suggest that LDL modified by glycation and oxidation may contribute to the development of accelerated atherosclerosis in the presence of diabetes, a process that may be prevented by increased vascular NO availability.

AB - Low-density lipoprotein (LDL) may undergo more glycation or oxidation in patients with diabetes mellitus than in nondiabetic subjects. We investigated whether glycoxidized LDL (goLDL) induces monocyte chemoattractant protein-1 (MCP-1) mRNA expression through activation of nuclear factor-kappaB (NFκB), and determined the effect of nitric oxide (NO) on MCP-1 mRNA expression in human umbilical vein endothelial cells (HUVEC). Oxidized (oxLDL) or goLDL enhanced MCP-1 mRNA expression in HUVEC, and preincubation with NOR3, a NO donor, abrogated such stimulation. goLDL increased NFκB-DNA binding activity in HUVEC and this effect was also suppressed by NOR3. We measured lysophosphatidylcholine (lyso-PC) contents in modified LDL using electrospray ionization liquid chromatography/mass spectrometry (LC/MS) to identify its molecular species. MCP-1 mRNA expression and NFκB activation correlated significantly with palmitoyl- and stearoyl-lyso-PC contents in LDL. Our results suggest that LDL modified by glycation and oxidation may contribute to the development of accelerated atherosclerosis in the presence of diabetes, a process that may be prevented by increased vascular NO availability.

UR - http://www.scopus.com/inward/record.url?scp=0036739001&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036739001&partnerID=8YFLogxK

U2 - 10.1053/meta.2002.34703

DO - 10.1053/meta.2002.34703

M3 - Article

VL - 51

SP - 1135

EP - 1142

JO - Metabolism: Clinical and Experimental

JF - Metabolism: Clinical and Experimental

SN - 0026-0495

IS - 9

ER -