Gonadotropic regulation of circadian clockwork in rat granulosa cells

Pei Jian He, Masami Hirata, Nobuhiko Yamauchi, Seiichi Hashimoto, Masa Aki Hattori

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The circadian clock is responsible for the generation of circadian rhythms in hormonal secretion and metabolism. These peripheral clocks could be reset by various cues in order to adapt to environmental variations. The ovary can be characterized as having highly dynamic physiology regulated by gonadotropins. Here, we aimed to address the status of circadian clock in the ovary, and to explore how gonadotropins could regulate clockwork in granulosa cells (GCs). To this end, we mainly utilized the immunohistochemistry, RT-PCR, and real-time monitoring of gene expression methods. PER1 protein was constantly abundant across the daily cycle in the GCs of immature ovaries. In contrast, PER1 protein level was obviously cyclic through the circadian cycle in the luteal cells of pubertal ovaries. In addition, both FSH and LH induced Per1 expression in cultured immature and mature GCs, respectively. The promoter analysis revealed that the Per1 expression was mediated by the cAMP response element binding protein. In cultured transgenic GCs, both FSH and LH also induced the circadian oscillation of Per2. However, the Per2 oscillation promoted by FSH quickly dampened within only one cycle, whereas the Per2 oscillation promoted by LH was persistently maintained. Collectively, these findings strongly suggest that both FSH and LH play an important role in regulating circadian clock in the ovary; however, they might exert differential actions on the clockwork in vivo due to each specific role within ovarian physiology.

Original languageEnglish
Pages (from-to)111-118
Number of pages8
JournalMolecular and cellular biochemistry
Volume302
Issue number1-2
DOIs
Publication statusPublished - Aug 1 2007

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Granulosa Cells
Rats
Clocks
Ovary
Circadian Clocks
Physiology
Gonadotropins
Cyclic AMP Response Element-Binding Protein
Luteal Cells
Metabolism
Gene expression
Gene Expression Profiling
Circadian Rhythm
Proteins
Cues
Real-Time Polymerase Chain Reaction
Immunohistochemistry
Monitoring

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Gonadotropic regulation of circadian clockwork in rat granulosa cells. / He, Pei Jian; Hirata, Masami; Yamauchi, Nobuhiko; Hashimoto, Seiichi; Hattori, Masa Aki.

In: Molecular and cellular biochemistry, Vol. 302, No. 1-2, 01.08.2007, p. 111-118.

Research output: Contribution to journalArticle

He, Pei Jian ; Hirata, Masami ; Yamauchi, Nobuhiko ; Hashimoto, Seiichi ; Hattori, Masa Aki. / Gonadotropic regulation of circadian clockwork in rat granulosa cells. In: Molecular and cellular biochemistry. 2007 ; Vol. 302, No. 1-2. pp. 111-118.
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