GPC-1, a G protein γ-subunit, regulates olfactory adaptation in Caenorhabditis elegans

Koji Yamada, Takaaki Hirotsu, Masahiro Matsuki, Hirofumi Kunitomo, Yuichi Iino

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Abstract

Caenorhabditis elegans genome carries two Gγ genes, gpc-1 and gpc-2, and two Gb genes, gpb-1 and gpb-2. Of these, gpc-2 and gpb-1 are expressed ubiquitously and are essential for viability. Through a genetic screen, we identified gpc-1 as essential for olfactory adaptation. While wild-type animals show decreased chemotaxis to the odorant benzaldehyde after a short preexposure to the odorant, gpc-1 mutants are still attracted to the odorant after the same preexposure. Cell-specific rescue experiments show that gpc-1 acts in the AWC olfactory neurons. Coexpression of GPC-1 and GPB-1, but not GPB-2, caused enhanced adaptation, indicating that GPC-1 may act with GPB-1. On the other hand, knock down of gpc-2 by cell-targeted RNAi caused reduced chemotaxis to the odorant in unadapted animals, indicating that GPC-2 mainly act for olfactory sensation and the two Gγ's have differential functions. Nonetheless, overexpression of gpc-2 in AWC neurons rescued the adaptation defects of gpc-1 mutants, suggesting partially overlapping functions of the two Gγ's. We further tested genetic interaction of gpc-1 with several other genes involved in olfactory adaptation. Our analyses place goα-1 Goa and let-60 Ras in parallel to gpc-1. In contrast, a gain-of-function mutation in egl-30 Gqα was epistatic to gpc-1, suggesting the possibility that gpc-1 Gγ may act upstream of egl-30 Gqα.

Original languageEnglish
Pages (from-to)1347-1357
Number of pages11
JournalGenetics
Volume181
Issue number4
DOIs
Publication statusPublished - Apr 1 2009

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All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Yamada, K., Hirotsu, T., Matsuki, M., Kunitomo, H., & Iino, Y. (2009). GPC-1, a G protein γ-subunit, regulates olfactory adaptation in Caenorhabditis elegans. Genetics, 181(4), 1347-1357. https://doi.org/10.1534/genetics.108.099002