Granulocyte Colony-stimulating Factor Receptors on Human Acute Leukemia

Biphenotypic Leukemic Cells Possess Granulocyte Colony-stimulating Factor Receptors

Kazuya Shimoda, Seiichi Okamura, Naoki Harada, Wataru Ikematsu, Seiji Kondo, Chiyuki Kawasaki, Takeshi Tanaka, Tetsuya Etou, Koichi Akashi, Takashi Okamura, Tsunefumi Shibuya, Mine Harada, Yoshiyuki Niho

Research output: Contribution to journalArticle

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Abstract

Granulocyte colony-stimulating factor (G-CSF) receptors on the gated leukemic blast cells from newly diagnosed patients with acute leukemia or crisis of chronic myelogenous leukemia were investigated using flow cytometric detection. Surface marker analysis and cytochemical studies were conducted simultaneously to characterize the blast cells. Among 24 leukemia cases examined, G-CSF receptor-positive blast cells were detected in all 11 cases of acute myeloblastic leukemia even though the percentage range of positive cells was widely variable. On the other hand, they were not detected on the blast cells from patients with peroxidase-negative acute lymphoblastic leukemia with no myeloid surface antigens. However, G-CSF receptors were demonstrated in significant amounts on blast cells from 5 of 8 cases of peroxidase-negative acute leukemia expressing both myeloid and lymphoid surface antigens (biphenotypic leukemia). The percentage of blast cells positive for G-CSF receptors was significantly smaller in biphenotypic cases [33 ± 14% (SD)1 than in acute myeloblasts leukemia cases (65 ± 22%] (P < 0.01). The percentage expression of CD 13 antigen by blast cells was significantly related to their percentage positivity for G-CSF receptors (rs = 0.50, P < 0.05). These findings indicate that the distribution of flow cytometrically detectable G-CSF receptors on leukemic cells possessing myeloid characteristics may be related to the maturation process.

Original languageEnglish
Pages (from-to)3052-3055
Number of pages4
JournalCancer Research
Volume52
Issue number11
Publication statusPublished - Jan 1 1992

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Biphenotypic Acute Leukemia
Granulocyte Colony-Stimulating Factor Receptors
Leukemia
Surface Antigens
Peroxidase
CD Antigens
Granulocyte Precursor Cells
Myeloid Cells
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Acute Myeloid Leukemia

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Shimoda, K., Okamura, S., Harada, N., Ikematsu, W., Kondo, S., Kawasaki, C., ... Niho, Y. (1992). Granulocyte Colony-stimulating Factor Receptors on Human Acute Leukemia: Biphenotypic Leukemic Cells Possess Granulocyte Colony-stimulating Factor Receptors. Cancer Research, 52(11), 3052-3055.

Granulocyte Colony-stimulating Factor Receptors on Human Acute Leukemia : Biphenotypic Leukemic Cells Possess Granulocyte Colony-stimulating Factor Receptors. / Shimoda, Kazuya; Okamura, Seiichi; Harada, Naoki; Ikematsu, Wataru; Kondo, Seiji; Kawasaki, Chiyuki; Tanaka, Takeshi; Etou, Tetsuya; Akashi, Koichi; Okamura, Takashi; Shibuya, Tsunefumi; Harada, Mine; Niho, Yoshiyuki.

In: Cancer Research, Vol. 52, No. 11, 01.01.1992, p. 3052-3055.

Research output: Contribution to journalArticle

Shimoda, K, Okamura, S, Harada, N, Ikematsu, W, Kondo, S, Kawasaki, C, Tanaka, T, Etou, T, Akashi, K, Okamura, T, Shibuya, T, Harada, M & Niho, Y 1992, 'Granulocyte Colony-stimulating Factor Receptors on Human Acute Leukemia: Biphenotypic Leukemic Cells Possess Granulocyte Colony-stimulating Factor Receptors', Cancer Research, vol. 52, no. 11, pp. 3052-3055.
Shimoda, Kazuya ; Okamura, Seiichi ; Harada, Naoki ; Ikematsu, Wataru ; Kondo, Seiji ; Kawasaki, Chiyuki ; Tanaka, Takeshi ; Etou, Tetsuya ; Akashi, Koichi ; Okamura, Takashi ; Shibuya, Tsunefumi ; Harada, Mine ; Niho, Yoshiyuki. / Granulocyte Colony-stimulating Factor Receptors on Human Acute Leukemia : Biphenotypic Leukemic Cells Possess Granulocyte Colony-stimulating Factor Receptors. In: Cancer Research. 1992 ; Vol. 52, No. 11. pp. 3052-3055.
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abstract = "Granulocyte colony-stimulating factor (G-CSF) receptors on the gated leukemic blast cells from newly diagnosed patients with acute leukemia or crisis of chronic myelogenous leukemia were investigated using flow cytometric detection. Surface marker analysis and cytochemical studies were conducted simultaneously to characterize the blast cells. Among 24 leukemia cases examined, G-CSF receptor-positive blast cells were detected in all 11 cases of acute myeloblastic leukemia even though the percentage range of positive cells was widely variable. On the other hand, they were not detected on the blast cells from patients with peroxidase-negative acute lymphoblastic leukemia with no myeloid surface antigens. However, G-CSF receptors were demonstrated in significant amounts on blast cells from 5 of 8 cases of peroxidase-negative acute leukemia expressing both myeloid and lymphoid surface antigens (biphenotypic leukemia). The percentage of blast cells positive for G-CSF receptors was significantly smaller in biphenotypic cases [33 ± 14{\%} (SD)1 than in acute myeloblasts leukemia cases (65 ± 22{\%}] (P < 0.01). The percentage expression of CD 13 antigen by blast cells was significantly related to their percentage positivity for G-CSF receptors (rs = 0.50, P < 0.05). These findings indicate that the distribution of flow cytometrically detectable G-CSF receptors on leukemic cells possessing myeloid characteristics may be related to the maturation process.",
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AU - Harada, Naoki

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AU - Etou, Tetsuya

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