Green tea polyphenol egcg upregulates tollip expression by suppressing ELF-1 expression

Motofumi Kumazoe, Mai Yamashita, Yuki Nakamura, Kanako Takamatsu, Jaehoon Bae, Shuya Yamashita, Shuhei Yamada, Hiroaki Onda, Takashi Nojiri, Kenji Kangawa, Hirofumi Tachibana

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

TLR signaling is critical to innate immune system regulation; however, aberrant TLR signaling is involved in several diseases, including insulin resistance, Alzheimer's disease, and tumor metastasis. Moreover, a recent study found that TLR-4 signaling pathway inhibition might be a target for the suppression of chronic inflammatory disorders. In this article, we show that the green tea polyphenol epigallocatechin-3-O-gallate (EGCG) increases the expression of Toll interacting protein, a strong inhibitor of TLR4 signaling, by suppressing the expression of E74-like ETS transcription factor 1 (Elf-1). A mechanistic study revealed that EGCG suppressed Elf-1 expression via protein phosphatase 2A/cyclic GMP (cGMP)-dependent mechanisms. We also confirmed that orally administered EGCG and a cGMP inducer upregulated Toll interacting protein expression, increased intracellular levels of cGMP in macrophages, and suppressed Elf-1 expression. These data support EGCG and a cGMP inducer as potential candidate suppressors of TLR4 signaling.

Original languageEnglish
Pages (from-to)3261-3269
Number of pages9
JournalJournal of Immunology
Volume199
Issue number9
DOIs
Publication statusPublished - Nov 1 2017

Fingerprint

Cyclic GMP
Polyphenols
Tea
Up-Regulation
Transcription Factors
Protein Phosphatase 2
Insulin Resistance
Immune System
Alzheimer Disease
Proteins
Macrophages
Neoplasm Metastasis
epigallocatechin gallate
Neoplasms

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Green tea polyphenol egcg upregulates tollip expression by suppressing ELF-1 expression. / Kumazoe, Motofumi; Yamashita, Mai; Nakamura, Yuki; Takamatsu, Kanako; Bae, Jaehoon; Yamashita, Shuya; Yamada, Shuhei; Onda, Hiroaki; Nojiri, Takashi; Kangawa, Kenji; Tachibana, Hirofumi.

In: Journal of Immunology, Vol. 199, No. 9, 01.11.2017, p. 3261-3269.

Research output: Contribution to journalArticle

Kumazoe, M, Yamashita, M, Nakamura, Y, Takamatsu, K, Bae, J, Yamashita, S, Yamada, S, Onda, H, Nojiri, T, Kangawa, K & Tachibana, H 2017, 'Green tea polyphenol egcg upregulates tollip expression by suppressing ELF-1 expression', Journal of Immunology, vol. 199, no. 9, pp. 3261-3269. https://doi.org/10.4049/jimmunol.1601822
Kumazoe M, Yamashita M, Nakamura Y, Takamatsu K, Bae J, Yamashita S et al. Green tea polyphenol egcg upregulates tollip expression by suppressing ELF-1 expression. Journal of Immunology. 2017 Nov 1;199(9):3261-3269. https://doi.org/10.4049/jimmunol.1601822
Kumazoe, Motofumi ; Yamashita, Mai ; Nakamura, Yuki ; Takamatsu, Kanako ; Bae, Jaehoon ; Yamashita, Shuya ; Yamada, Shuhei ; Onda, Hiroaki ; Nojiri, Takashi ; Kangawa, Kenji ; Tachibana, Hirofumi. / Green tea polyphenol egcg upregulates tollip expression by suppressing ELF-1 expression. In: Journal of Immunology. 2017 ; Vol. 199, No. 9. pp. 3261-3269.
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