Green tea polyphenol epigallocatechin-3-gallate signaling pathway through 67-kDa laminin receptor

Daisuke Umeda, Satomi Yano, Koji Yamada, Hirofumi Tachibana

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

(-)-Epigallocatechin-3-gallate (EGCG), the principal polyphenol in green tea, has been shown to be a potent chemopreventive agent. Recently, 67-kDa laminin receptor (67LR) has been identified as a cell surface receptor for EGCG that mediates the anticancer activity of EGCG. Indeed, expression of 67LR confers EGCG responsiveness to tumor cells; however, the molecular basis for the anticancer activity of EGCG in vivo is not entirely understood. Here we show that (i) using a direct genetic screen, eukaryotic translation elongation factor 1A (eEF1A) is identified as a component responsible for the anticancer activity of EGCG; (ii) through both eEF1A and 67LR, EGCG induces the dephosphorylation of myosin phosphatase targeting subunit 1 (MYPT1) at Thr-696 and activates myosin phosphatase; and (iii) silencing of 67LR, eEF1A, or MYPT1 in tumor cells results in abrogation of EGCG-induced tumor growth inhibition in vivo. Additionally, we found that eEF1A is up-regulated by EGCG through 67LR. Overall, these findings implicate both eEF1A and MYPT1 in EGCG signaling for cancer prevention through 67LR.

Original languageEnglish
Pages (from-to)3050-3058
Number of pages9
JournalJournal of Biological Chemistry
Volume283
Issue number6
DOIs
Publication statusPublished - Feb 8 2008

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Laminin Receptors
Polyphenols
Tea
Peptide Elongation Factors
Myosin-Light-Chain Phosphatase
Tumors
Neoplasms
Cells
epigallocatechin gallate
Cell Surface Receptors

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Green tea polyphenol epigallocatechin-3-gallate signaling pathway through 67-kDa laminin receptor. / Umeda, Daisuke; Yano, Satomi; Yamada, Koji; Tachibana, Hirofumi.

In: Journal of Biological Chemistry, Vol. 283, No. 6, 08.02.2008, p. 3050-3058.

Research output: Contribution to journalArticle

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