Grouping of isolates in AG 2 of Rhizoctonia solani by total cellular fatty acid analysis

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Abstract

Total-cellular fatty acid compositions of 34 isolates of Rhizoctonia solani belonging to intraspecific groups (ISGs) of anastomosis group (AG) 2, i.e., AG 2-1, AG 2-2 IIIB (mat rush), AG 2-2 IV (sugar beet), AG 2-2 LP (turfgrass), and AG 23 (soybean), were compared. The major fatty acids identified were palmitic, stearic, and oleic acids. Principal component analysis based on the percentage composition of total cellular fatty acids revealed consistently low variability among isolates of a single ISG of AG 2. Average linkage cluster analysis showed that isolates obtained from turfgrass representing a newly proposed group, AG 2-2 LP, were differentiated from other AG 2 ISGs. Isolates of another newly proposed group AG 2-3, from diseased soybean were also closely related to AG 2-1 and AG 2-2 IIIB but distinguishable from the AG 2-1 and AG 2-2 LP isolates by the average linkage cluster analysis. These results suggested that the percentage composition of total-cellular fatty acids is a distinct characteristic for the five ISGs belonging to AG 2, and fatty acid analysis is useful for the differentiation and characterization of these ISGs of AG 2 in R. solani.

Original languageEnglish
Pages (from-to)35-39
Number of pages5
JournalMycoscience
Volume40
Issue number1
DOIs
Publication statusPublished - Feb 15 1999

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Thanatephorus cucumeris
fatty acid
fatty acids
turf grasses
cluster analysis
soybean
soybeans
stearic acid
palmitic acid
sugar beet
oleic acid
principal component analysis
fatty acid composition
analysis
acid

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics

Cite this

Grouping of isolates in AG 2 of Rhizoctonia solani by total cellular fatty acid analysis. / Masaru, Matsumoto.

In: Mycoscience, Vol. 40, No. 1, 15.02.1999, p. 35-39.

Research output: Contribution to journalArticle

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