TY - JOUR
T1 - Growth comparison between lymphocyte bw5147 t cells and el4 t cells using atmospheric oxygen plasma irradiation
AU - Subaedah, Sitti
AU - Uematsu, Haruka
AU - Hayashi, Nobuya
N1 - Funding Information:
The authors would like to express their gratitude to Professor Yoshio Yamashita at Saga University for fruitful discussion. This work was supported by JSPS KAKENHI (Grant No. JP20H01892).
Publisher Copyright:
© 2021 by Begell House, Inc. www.begellhouse.com.
PY - 2021
Y1 - 2021
N2 - The activation of T cells (EL4 and BW5147) was examined when they were sensitized with monoclonal antibodies against CD3/CD28 followed by irradiation with atmospheric oxygen plasma. The proliferation of EL4 and BW5147 T cells sensitized by anti-CD3/CD28 was evaluated as a function of the duration of oxygen plasma irradiation. Plasma irradiation significantly promoted cell proliferation; when plasma irradiation was applied to sensitized T cells for 50 s, cell numbers increased 1.8 times over 24 h compared with cells that were not exposed to irradiation. We compared IFN-gamma production between EL4 T cells and BW5147 T cells after plasma irradiation. The results indicated that the IFN-gamma production in EL4 T cells tends to increase under most conditions compared with controls (without plasma irradiation), while in BW5147 T cells, due to few active oxygen species produced from plasma irradiation, IFN-gamma tends not to increase for any irradiation periods compared with the control. In addition, gene expression analysis of EL4 T cells indicated that MAPK-related ERK genes and IFNG genes were upregulated by oxygen plasma irradiation, which proves that plasma irradiation enhances immune function.
AB - The activation of T cells (EL4 and BW5147) was examined when they were sensitized with monoclonal antibodies against CD3/CD28 followed by irradiation with atmospheric oxygen plasma. The proliferation of EL4 and BW5147 T cells sensitized by anti-CD3/CD28 was evaluated as a function of the duration of oxygen plasma irradiation. Plasma irradiation significantly promoted cell proliferation; when plasma irradiation was applied to sensitized T cells for 50 s, cell numbers increased 1.8 times over 24 h compared with cells that were not exposed to irradiation. We compared IFN-gamma production between EL4 T cells and BW5147 T cells after plasma irradiation. The results indicated that the IFN-gamma production in EL4 T cells tends to increase under most conditions compared with controls (without plasma irradiation), while in BW5147 T cells, due to few active oxygen species produced from plasma irradiation, IFN-gamma tends not to increase for any irradiation periods compared with the control. In addition, gene expression analysis of EL4 T cells indicated that MAPK-related ERK genes and IFNG genes were upregulated by oxygen plasma irradiation, which proves that plasma irradiation enhances immune function.
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U2 - 10.1615/PLASMAMED.2021038540
DO - 10.1615/PLASMAMED.2021038540
M3 - Article
AN - SCOPUS:85119610972
VL - 11
SP - 43
EP - 54
JO - Plasma Medicine
JF - Plasma Medicine
SN - 1947-5764
IS - 2
ER -