Growth-dependent chemical and mechanical properties of cuticular membranes from leaves of Sonneratia alba

Yuki Takahashi, Shuntaro Tsubaki, Masahiro Sakamoto, Shin Watanabe, Jun Ichi Azuma

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24 Citations (Scopus)

Abstract

Chemical and mechanical properties of the leaf cuticular membranes (CMs) of a mangrove, Sonneratia alba J. Smith, were analysed at various leaf development stages to evaluate their tolerance to environmental stress. Our analyses demonstrate that the CMs from leaves of S. alba at different growth stages are generally rich in wax (21.5-25.7%) and cutin (52.4-63.4%) which rapidly accumulate at the early stages of leaf growth, while cutan (4.3-10.3%) and polysaccharide (2.3-7.7%) continuously accumulate throughout growth. Immature CMs are physically weak and highly viscoelastic. However, CMs become strengthened and stiffened during leaf expansion and maturation (by factors of about 1.5 and 2.4, respectively) while their flexibility decreases (68-83% decrease). Finally, the CMs lose their strength at the senescent stage (30-43% decreasement). Correlation analysis between chemical composition and mechanical properties revealed that the cutin matrix is mainly responsible for the high viscoelastic properties of CMs, while wax, cutan and polysaccharide contributed to their elasticity. Wax also affected the strength of the CMs, whereas cutan and polysaccharide showed rigidizing effect. Rapid accumulation of wax and cutin in the CMs after bud burst followed by the mechanical supports of cutan and polysaccharide in an isolateral manner contributed to the remarkable environmental tolerance of S. alba. Highlighting statement of this work. Importance of chemical and mechanical supports of the leaf cuticular membranes (CMs) of a mangrove, Sonneratia alba, for tolerance to environmental stress was analyzed by using five pairs of leaves at different growth stages. Highly viscoelasticity and low physical strength observed in the CMs from immature leaves was mainly ascribed to the predominant CM components, cutin. As leaf expansion and maturation progress, the CMs are strengthened and stiffened (about 1.5 fold and 2.4 fold, respectively) together with the loss of flexibility (68-83% decrease) by the contribution of cuticular wax, cutan and polysaccharide, and finally the physical strength was lost at the aged and almost defoliating leaves. Rapid accumulation of wax and cutin in the CMs after bud-burst followed by the mechanical supports of cutan and polysaccharide in an isolateral manner contributed to the remarkable environmental tolerance of S. alba.

Original languageEnglish
Pages (from-to)1201-1210
Number of pages10
JournalPlant, Cell and Environment
Volume35
Issue number7
DOIs
Publication statusPublished - Jul 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

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