Growth factor induced proliferation, migration, and lumen formation of rat endometrial epithelial cells in vitro

Md Rashedul Islam, Kazuki Yamagami, Yuka Yoshii, Nobuhiko Yamauchi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Endometrial modulation is essential for the preservation of normal uterine physiology, and this modulation is driven by a number of growth factors. The present study investigated the mitogenic, motogenic, and morphogenic effects of epidermal growth factor (EGF) and hepatocyte growth factor (HGF) on rat endometrial epithelial (REE) cells. The REE cells were isolated and cultured and then characterized based on their morphology and their expression of epithelial cell markers. The MTT assay revealed that EGF and HGF induce proliferation of REE cells. Consistent with increased proliferation, we found that the cell cycle regulatory factor Cyclin D1 was also upregulated upon EGF and HGF addition. REE cell migration was prompted by EGF, as observed with the Oris Cell Migration Assay. The morphogenic impact of growth factors on REE cells was studied in a three-dimensional BD Matrigel cell culture system, wherein these growth factors also increased the frequency of lumen formation. In summary, we show that EGF and HGF have a stimulatory effect on REE cells, promoting proliferation, cell migration, and lumen formation. Our findings provide important insights that further the understanding of endometrial regeneration and its regulation.

Original languageEnglish
Pages (from-to)271-278
Number of pages8
JournalJournal of Reproduction and Development
Volume62
Issue number3
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

growth factors
epithelial cells
epidermal growth factor
hepatocyte growth factor
rats
cell movement
cyclins
assays
cell cycle
cell culture
physiology

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

Growth factor induced proliferation, migration, and lumen formation of rat endometrial epithelial cells in vitro. / Islam, Md Rashedul; Yamagami, Kazuki; Yoshii, Yuka; Yamauchi, Nobuhiko.

In: Journal of Reproduction and Development, Vol. 62, No. 3, 01.01.2016, p. 271-278.

Research output: Contribution to journalArticle

@article{3ff91786df084e55be7cba84113608a1,
title = "Growth factor induced proliferation, migration, and lumen formation of rat endometrial epithelial cells in vitro",
abstract = "Endometrial modulation is essential for the preservation of normal uterine physiology, and this modulation is driven by a number of growth factors. The present study investigated the mitogenic, motogenic, and morphogenic effects of epidermal growth factor (EGF) and hepatocyte growth factor (HGF) on rat endometrial epithelial (REE) cells. The REE cells were isolated and cultured and then characterized based on their morphology and their expression of epithelial cell markers. The MTT assay revealed that EGF and HGF induce proliferation of REE cells. Consistent with increased proliferation, we found that the cell cycle regulatory factor Cyclin D1 was also upregulated upon EGF and HGF addition. REE cell migration was prompted by EGF, as observed with the Oris Cell Migration Assay. The morphogenic impact of growth factors on REE cells was studied in a three-dimensional BD Matrigel cell culture system, wherein these growth factors also increased the frequency of lumen formation. In summary, we show that EGF and HGF have a stimulatory effect on REE cells, promoting proliferation, cell migration, and lumen formation. Our findings provide important insights that further the understanding of endometrial regeneration and its regulation.",
author = "Islam, {Md Rashedul} and Kazuki Yamagami and Yuka Yoshii and Nobuhiko Yamauchi",
year = "2016",
month = "1",
day = "1",
doi = "10.1262/jrd.2015-158",
language = "English",
volume = "62",
pages = "271--278",
journal = "Journal of Reproduction and Development",
issn = "0916-8818",
publisher = "Japanese Society of Animal Reproduction (JSAR)",
number = "3",

}

TY - JOUR

T1 - Growth factor induced proliferation, migration, and lumen formation of rat endometrial epithelial cells in vitro

AU - Islam, Md Rashedul

AU - Yamagami, Kazuki

AU - Yoshii, Yuka

AU - Yamauchi, Nobuhiko

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Endometrial modulation is essential for the preservation of normal uterine physiology, and this modulation is driven by a number of growth factors. The present study investigated the mitogenic, motogenic, and morphogenic effects of epidermal growth factor (EGF) and hepatocyte growth factor (HGF) on rat endometrial epithelial (REE) cells. The REE cells were isolated and cultured and then characterized based on their morphology and their expression of epithelial cell markers. The MTT assay revealed that EGF and HGF induce proliferation of REE cells. Consistent with increased proliferation, we found that the cell cycle regulatory factor Cyclin D1 was also upregulated upon EGF and HGF addition. REE cell migration was prompted by EGF, as observed with the Oris Cell Migration Assay. The morphogenic impact of growth factors on REE cells was studied in a three-dimensional BD Matrigel cell culture system, wherein these growth factors also increased the frequency of lumen formation. In summary, we show that EGF and HGF have a stimulatory effect on REE cells, promoting proliferation, cell migration, and lumen formation. Our findings provide important insights that further the understanding of endometrial regeneration and its regulation.

AB - Endometrial modulation is essential for the preservation of normal uterine physiology, and this modulation is driven by a number of growth factors. The present study investigated the mitogenic, motogenic, and morphogenic effects of epidermal growth factor (EGF) and hepatocyte growth factor (HGF) on rat endometrial epithelial (REE) cells. The REE cells were isolated and cultured and then characterized based on their morphology and their expression of epithelial cell markers. The MTT assay revealed that EGF and HGF induce proliferation of REE cells. Consistent with increased proliferation, we found that the cell cycle regulatory factor Cyclin D1 was also upregulated upon EGF and HGF addition. REE cell migration was prompted by EGF, as observed with the Oris Cell Migration Assay. The morphogenic impact of growth factors on REE cells was studied in a three-dimensional BD Matrigel cell culture system, wherein these growth factors also increased the frequency of lumen formation. In summary, we show that EGF and HGF have a stimulatory effect on REE cells, promoting proliferation, cell migration, and lumen formation. Our findings provide important insights that further the understanding of endometrial regeneration and its regulation.

UR - http://www.scopus.com/inward/record.url?scp=84975082481&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84975082481&partnerID=8YFLogxK

U2 - 10.1262/jrd.2015-158

DO - 10.1262/jrd.2015-158

M3 - Article

C2 - 26946922

AN - SCOPUS:84975082481

VL - 62

SP - 271

EP - 278

JO - Journal of Reproduction and Development

JF - Journal of Reproduction and Development

SN - 0916-8818

IS - 3

ER -