Growth induction of rat primary hepatocytes using antisense oligonucleotides

Ryuji Hamamoto, Hirofumi Seko, Ryosuke Kamimura, Keisuke Yamada, Kiyohito Murai, Masamichi Kamihira, Shinji Iijima

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Abstract

We examined growth control of adult and fetal hepatocytes by regulating the expression of cell-cycle-related proteins using antisense S- oligonucleotides to tumor suppressors retinoblastoma (RB) protein and p53, and cyclin-dependent kinase (CDK) inhibitors p21 and p27. The protein expression in both adult and fetal hepatocytes was significantly suppressed with the addition of corresponding antisense oligonucleotides at a concentration of 2.5 μM. For the evaluation of growth, 3H-thymidine incorporation and DNA content were measured and the results demonstrated that all the antisense oligonucleotides had growth-promoting effects and the promoting potential was equivalent or slightly greater than that with the addition of hepatocyte growth factor (HGF) (10 ng/ml). The growth-promoting effect of the antisense oligonucleotides was enhanced by HGF in both adult and fetal hepatocyte cultures, and the effects on hepatocyte growth were also observed in a suspension culture.

Original languageEnglish
Pages (from-to)310-315
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume88
Issue number3
DOIs
Publication statusPublished - Sep 1999
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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