Abstract
We examined growth control of adult and fetal hepatocytes by regulating the expression of cell-cycle-related proteins using antisense S- oligonucleotides to tumor suppressors retinoblastoma (RB) protein and p53, and cyclin-dependent kinase (CDK) inhibitors p21 and p27. The protein expression in both adult and fetal hepatocytes was significantly suppressed with the addition of corresponding antisense oligonucleotides at a concentration of 2.5 μM. For the evaluation of growth, 3H-thymidine incorporation and DNA content were measured and the results demonstrated that all the antisense oligonucleotides had growth-promoting effects and the promoting potential was equivalent or slightly greater than that with the addition of hepatocyte growth factor (HGF) (10 ng/ml). The growth-promoting effect of the antisense oligonucleotides was enhanced by HGF in both adult and fetal hepatocyte cultures, and the effects on hepatocyte growth were also observed in a suspension culture.
| Original language | English |
|---|---|
| Pages (from-to) | 310-315 |
| Number of pages | 6 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 88 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jan 1 1999 |
| Externally published | Yes |
Fingerprint
All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
Cite this
Growth induction of rat primary hepatocytes using antisense oligonucleotides. / Hamamoto, Ryuji; Seko, Hirofumi; Kamimura, Ryosuke; Yamada, Keisuke; Murai, Kiyohito; Kamihira, Masamichi; Iijima, Shinji.
In: Journal of Bioscience and Bioengineering, Vol. 88, No. 3, 01.01.1999, p. 310-315.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Growth induction of rat primary hepatocytes using antisense oligonucleotides
AU - Hamamoto, Ryuji
AU - Seko, Hirofumi
AU - Kamimura, Ryosuke
AU - Yamada, Keisuke
AU - Murai, Kiyohito
AU - Kamihira, Masamichi
AU - Iijima, Shinji
PY - 1999/1/1
Y1 - 1999/1/1
N2 - We examined growth control of adult and fetal hepatocytes by regulating the expression of cell-cycle-related proteins using antisense S- oligonucleotides to tumor suppressors retinoblastoma (RB) protein and p53, and cyclin-dependent kinase (CDK) inhibitors p21 and p27. The protein expression in both adult and fetal hepatocytes was significantly suppressed with the addition of corresponding antisense oligonucleotides at a concentration of 2.5 μM. For the evaluation of growth, 3H-thymidine incorporation and DNA content were measured and the results demonstrated that all the antisense oligonucleotides had growth-promoting effects and the promoting potential was equivalent or slightly greater than that with the addition of hepatocyte growth factor (HGF) (10 ng/ml). The growth-promoting effect of the antisense oligonucleotides was enhanced by HGF in both adult and fetal hepatocyte cultures, and the effects on hepatocyte growth were also observed in a suspension culture.
AB - We examined growth control of adult and fetal hepatocytes by regulating the expression of cell-cycle-related proteins using antisense S- oligonucleotides to tumor suppressors retinoblastoma (RB) protein and p53, and cyclin-dependent kinase (CDK) inhibitors p21 and p27. The protein expression in both adult and fetal hepatocytes was significantly suppressed with the addition of corresponding antisense oligonucleotides at a concentration of 2.5 μM. For the evaluation of growth, 3H-thymidine incorporation and DNA content were measured and the results demonstrated that all the antisense oligonucleotides had growth-promoting effects and the promoting potential was equivalent or slightly greater than that with the addition of hepatocyte growth factor (HGF) (10 ng/ml). The growth-promoting effect of the antisense oligonucleotides was enhanced by HGF in both adult and fetal hepatocyte cultures, and the effects on hepatocyte growth were also observed in a suspension culture.
UR - http://www.scopus.com/inward/record.url?scp=0033199965&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033199965&partnerID=8YFLogxK
U2 - 10.1016/S1389-1723(00)80015-8
DO - 10.1016/S1389-1723(00)80015-8
M3 - Article
VL - 88
SP - 310
EP - 315
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 3
ER -