TY - JOUR
T1 - Growth induction of rat primary hepatocytes using antisense oligonucleotides
AU - Hamamoto, Ryuji
AU - Seko, Hirofumi
AU - Kamimura, Ryosuke
AU - Yamada, Keisuke
AU - Murai, Kiyohito
AU - Kamihira, Masamichi
AU - Iijima, Shinji
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research from Terumo Science Foundation. R.H. was supported by a Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists.
PY - 1999/9
Y1 - 1999/9
N2 - We examined growth control of adult and fetal hepatocytes by regulating the expression of cell-cycle-related proteins using antisense S- oligonucleotides to tumor suppressors retinoblastoma (RB) protein and p53, and cyclin-dependent kinase (CDK) inhibitors p21 and p27. The protein expression in both adult and fetal hepatocytes was significantly suppressed with the addition of corresponding antisense oligonucleotides at a concentration of 2.5 μM. For the evaluation of growth, 3H-thymidine incorporation and DNA content were measured and the results demonstrated that all the antisense oligonucleotides had growth-promoting effects and the promoting potential was equivalent or slightly greater than that with the addition of hepatocyte growth factor (HGF) (10 ng/ml). The growth-promoting effect of the antisense oligonucleotides was enhanced by HGF in both adult and fetal hepatocyte cultures, and the effects on hepatocyte growth were also observed in a suspension culture.
AB - We examined growth control of adult and fetal hepatocytes by regulating the expression of cell-cycle-related proteins using antisense S- oligonucleotides to tumor suppressors retinoblastoma (RB) protein and p53, and cyclin-dependent kinase (CDK) inhibitors p21 and p27. The protein expression in both adult and fetal hepatocytes was significantly suppressed with the addition of corresponding antisense oligonucleotides at a concentration of 2.5 μM. For the evaluation of growth, 3H-thymidine incorporation and DNA content were measured and the results demonstrated that all the antisense oligonucleotides had growth-promoting effects and the promoting potential was equivalent or slightly greater than that with the addition of hepatocyte growth factor (HGF) (10 ng/ml). The growth-promoting effect of the antisense oligonucleotides was enhanced by HGF in both adult and fetal hepatocyte cultures, and the effects on hepatocyte growth were also observed in a suspension culture.
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U2 - 10.1016/S1389-1723(00)80015-8
DO - 10.1016/S1389-1723(00)80015-8
M3 - Article
C2 - 16232617
AN - SCOPUS:0033199965
VL - 88
SP - 310
EP - 315
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 3
ER -