Abstract
We examined growth control of adult and fetal hepatocytes by regulating the expression of cell-cycle-related proteins using antisense S- oligonucleotides to tumor suppressors retinoblastoma (RB) protein and p53, and cyclin-dependent kinase (CDK) inhibitors p21 and p27. The protein expression in both adult and fetal hepatocytes was significantly suppressed with the addition of corresponding antisense oligonucleotides at a concentration of 2.5 μM. For the evaluation of growth, 3H-thymidine incorporation and DNA content were measured and the results demonstrated that all the antisense oligonucleotides had growth-promoting effects and the promoting potential was equivalent or slightly greater than that with the addition of hepatocyte growth factor (HGF) (10 ng/ml). The growth-promoting effect of the antisense oligonucleotides was enhanced by HGF in both adult and fetal hepatocyte cultures, and the effects on hepatocyte growth were also observed in a suspension culture.
Original language | English |
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Pages (from-to) | 310-315 |
Number of pages | 6 |
Journal | Journal of Bioscience and Bioengineering |
Volume | 88 |
Issue number | 3 |
DOIs | |
Publication status | Published - Sep 1999 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology