Growth-phase dependent variation in photosynthetic activity and cellular protein expression profile in the harmful raphidophyte Chattonella antiqua

Xuchun Qiu, Yohei Shimasaki, Michito Tsuyama, Taku Yamada, Rie Kuwahara, Mayumi Kawaguchi, Masato Honda, Hiroaki Gunjikake, Rumana Tasmin, Motoyuki Shimizu, Yusuke Sato, Yoko Kato-Unoki, Takashi Nakashima, Tadashi Matsubara, Yasuhiro Yamasaki, Hirofumi Ichinose, Hiroyuki Wariishi, Tsuneo Honjo, Yuji Oshima

Research output: Contribution to journalArticle

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Abstract

This study investigated temporal variations in the potential maximum quantum yield of photosystem II (Fv/Fm ratio) and growth-phase dependent cellular protein expressions of Chattonella antiqua under laboratory conditions. Despite the culture conditions, significant positive correlations between the Fv/Fm ratio and daily growth rate were observed. Threshold Fv/Fm ratios associated with positive cell growth were calculated to be >0:44, >0:44, and >0:37, and those associated with active cell growth (growth rate >0:5 div. d-1) were >0:58, >0:60, and >0:49 under control culture, low nutrient and intense light conditions, respectively. Proteome profiles obtained by two-dimensional gel electrophoresis (2-DE) indicated that 42 protein spots were differentially expressed at various growth phases of C. antiqua, which indicates changes in cellular physiological status throughout the growth cycle, and suggests that oxygen evolving enhancer 1 and 2-cysteine peroxiredoxin play roles in maintaining the positive growth of C. antiqua.

Original languageEnglish
Pages (from-to)46-52
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume77
Issue number1
DOIs
Publication statusPublished - Feb 14 2013

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Cell growth
Growth
Proteins
Peroxiredoxins
Photosystem II Protein Complex
Quantum yield
Proteome
Electrophoresis
Nutrients
Cysteine
Gels
Oxygen
Electrophoresis, Gel, Two-Dimensional
Light
Food

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Growth-phase dependent variation in photosynthetic activity and cellular protein expression profile in the harmful raphidophyte Chattonella antiqua. / Qiu, Xuchun; Shimasaki, Yohei; Tsuyama, Michito; Yamada, Taku; Kuwahara, Rie; Kawaguchi, Mayumi; Honda, Masato; Gunjikake, Hiroaki; Tasmin, Rumana; Shimizu, Motoyuki; Sato, Yusuke; Kato-Unoki, Yoko; Nakashima, Takashi; Matsubara, Tadashi; Yamasaki, Yasuhiro; Ichinose, Hirofumi; Wariishi, Hiroyuki; Honjo, Tsuneo; Oshima, Yuji.

In: Bioscience, Biotechnology and Biochemistry, Vol. 77, No. 1, 14.02.2013, p. 46-52.

Research output: Contribution to journalArticle

Qiu, Xuchun ; Shimasaki, Yohei ; Tsuyama, Michito ; Yamada, Taku ; Kuwahara, Rie ; Kawaguchi, Mayumi ; Honda, Masato ; Gunjikake, Hiroaki ; Tasmin, Rumana ; Shimizu, Motoyuki ; Sato, Yusuke ; Kato-Unoki, Yoko ; Nakashima, Takashi ; Matsubara, Tadashi ; Yamasaki, Yasuhiro ; Ichinose, Hirofumi ; Wariishi, Hiroyuki ; Honjo, Tsuneo ; Oshima, Yuji. / Growth-phase dependent variation in photosynthetic activity and cellular protein expression profile in the harmful raphidophyte Chattonella antiqua. In: Bioscience, Biotechnology and Biochemistry. 2013 ; Vol. 77, No. 1. pp. 46-52.
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