Abstract
This study investigated temporal variations in the potential maximum quantum yield of photosystem II (Fv/Fm ratio) and growth-phase dependent cellular protein expressions of Chattonella antiqua under laboratory conditions. Despite the culture conditions, significant positive correlations between the Fv/Fm ratio and daily growth rate were observed. Threshold Fv/Fm ratios associated with positive cell growth were calculated to be >0:44, >0:44, and >0:37, and those associated with active cell growth (growth rate >0:5 div. d-1) were >0:58, >0:60, and >0:49 under control culture, low nutrient and intense light conditions, respectively. Proteome profiles obtained by two-dimensional gel electrophoresis (2-DE) indicated that 42 protein spots were differentially expressed at various growth phases of C. antiqua, which indicates changes in cellular physiological status throughout the growth cycle, and suggests that oxygen evolving enhancer 1 and 2-cysteine peroxiredoxin play roles in maintaining the positive growth of C. antiqua.
| Original language | English |
|---|---|
| Pages (from-to) | 46-52 |
| Number of pages | 7 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 77 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 14 2013 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry
Cite this
Growth-phase dependent variation in photosynthetic activity and cellular protein expression profile in the harmful raphidophyte Chattonella antiqua. / Qiu, Xuchun; Shimasaki, Yohei; Tsuyama, Michito; Yamada, Taku; Kuwahara, Rie; Kawaguchi, Mayumi; Honda, Masato; Gunjikake, Hiroaki; Tasmin, Rumana; Shimizu, Motoyuki; Sato, Yusuke; Kato-Unoki, Yoko; Nakashima, Takashi; Matsubara, Tadashi; Yamasaki, Yasuhiro; Ichinose, Hirofumi; Wariishi, Hiroyuki; Honjo, Tsuneo; Oshima, Yuji.
In: Bioscience, Biotechnology and Biochemistry, Vol. 77, No. 1, 14.02.2013, p. 46-52.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Growth-phase dependent variation in photosynthetic activity and cellular protein expression profile in the harmful raphidophyte Chattonella antiqua
AU - Qiu, Xuchun
AU - Shimasaki, Yohei
AU - Tsuyama, Michito
AU - Yamada, Taku
AU - Kuwahara, Rie
AU - Kawaguchi, Mayumi
AU - Honda, Masato
AU - Gunjikake, Hiroaki
AU - Tasmin, Rumana
AU - Shimizu, Motoyuki
AU - Sato, Yusuke
AU - Kato-Unoki, Yoko
AU - Nakashima, Takashi
AU - Matsubara, Tadashi
AU - Yamasaki, Yasuhiro
AU - Ichinose, Hirofumi
AU - Wariishi, Hiroyuki
AU - Honjo, Tsuneo
AU - Oshima, Yuji
PY - 2013/2/14
Y1 - 2013/2/14
N2 - This study investigated temporal variations in the potential maximum quantum yield of photosystem II (Fv/Fm ratio) and growth-phase dependent cellular protein expressions of Chattonella antiqua under laboratory conditions. Despite the culture conditions, significant positive correlations between the Fv/Fm ratio and daily growth rate were observed. Threshold Fv/Fm ratios associated with positive cell growth were calculated to be >0:44, >0:44, and >0:37, and those associated with active cell growth (growth rate >0:5 div. d-1) were >0:58, >0:60, and >0:49 under control culture, low nutrient and intense light conditions, respectively. Proteome profiles obtained by two-dimensional gel electrophoresis (2-DE) indicated that 42 protein spots were differentially expressed at various growth phases of C. antiqua, which indicates changes in cellular physiological status throughout the growth cycle, and suggests that oxygen evolving enhancer 1 and 2-cysteine peroxiredoxin play roles in maintaining the positive growth of C. antiqua.
AB - This study investigated temporal variations in the potential maximum quantum yield of photosystem II (Fv/Fm ratio) and growth-phase dependent cellular protein expressions of Chattonella antiqua under laboratory conditions. Despite the culture conditions, significant positive correlations between the Fv/Fm ratio and daily growth rate were observed. Threshold Fv/Fm ratios associated with positive cell growth were calculated to be >0:44, >0:44, and >0:37, and those associated with active cell growth (growth rate >0:5 div. d-1) were >0:58, >0:60, and >0:49 under control culture, low nutrient and intense light conditions, respectively. Proteome profiles obtained by two-dimensional gel electrophoresis (2-DE) indicated that 42 protein spots were differentially expressed at various growth phases of C. antiqua, which indicates changes in cellular physiological status throughout the growth cycle, and suggests that oxygen evolving enhancer 1 and 2-cysteine peroxiredoxin play roles in maintaining the positive growth of C. antiqua.
UR - http://www.scopus.com/inward/record.url?scp=84873563877&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84873563877&partnerID=8YFLogxK
U2 - 10.1271/bbb.120543
DO - 10.1271/bbb.120543
M3 - Article
C2 - 23291769
AN - SCOPUS:84873563877
VL - 77
SP - 46
EP - 52
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 1
ER -