Growth retardation and dyslymphopoiesis accompanied by G2/M arrest in APEX2-null mice

Yasuhito Ide, Daisuke Tsuchimoto, Yohei Tominaga, Manabu Nakashima, Takeshi Watanabe, Sakumi Kunihiko, Ohno Mizuki, Yusaku Nakabeppu

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

APEX2/APE2 is a secondary mammalian apurinic/apyrimidinic endonuclease that associates with proliferating cell nuclear antigen (PCNA), and the progression of S phase of the cell cycle is accompanied by its expression. To determine the biologic significance of APEX2, we established APEX2-null mice. These mice were about 80% the size of their wild-type littermates and exhibited a moderate dyshematopoiesis and a relatively severe defect in lymphopoiesis. A significant accumulation of both thymocytes and mitogen-stimulated splenocytes in G 2/M phase was seen in APEX2-null mice compared with the wild type, indicating that APEX2 is required for proper cell cycle progression of proliferating lymphocytes. Although APEX2-null mice exhibited an attenuated immune response against ovalbumin in comparison with wild-type mice, they produced both antiovalbumin immunoglobulin M (IgM) and IgG, indicating that class switch recombination can occur even in the absence of APEX2.

Original languageEnglish
Pages (from-to)4097-4103
Number of pages7
JournalBlood
Volume104
Issue number13
DOIs
Publication statusPublished - Dec 15 2004

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Cells
Lymphocytes
Endonucleases
Ovalbumin
Proliferating Cell Nuclear Antigen
Growth
Mitogens
Immunoglobulin M
Immunoglobulin G
Switches
Cell Cycle
Defects
DNA-(Apurinic or Apyrimidinic Site) Lyase
Lymphopoiesis
Thymocytes
S Phase
Cell Division
Genetic Recombination

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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Growth retardation and dyslymphopoiesis accompanied by G2/M arrest in APEX2-null mice. / Ide, Yasuhito; Tsuchimoto, Daisuke; Tominaga, Yohei; Nakashima, Manabu; Watanabe, Takeshi; Kunihiko, Sakumi; Mizuki, Ohno; Nakabeppu, Yusaku.

In: Blood, Vol. 104, No. 13, 15.12.2004, p. 4097-4103.

Research output: Contribution to journalArticle

Ide, Yasuhito ; Tsuchimoto, Daisuke ; Tominaga, Yohei ; Nakashima, Manabu ; Watanabe, Takeshi ; Kunihiko, Sakumi ; Mizuki, Ohno ; Nakabeppu, Yusaku. / Growth retardation and dyslymphopoiesis accompanied by G2/M arrest in APEX2-null mice. In: Blood. 2004 ; Vol. 104, No. 13. pp. 4097-4103.
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AU - Watanabe, Takeshi

AU - Kunihiko, Sakumi

AU - Mizuki, Ohno

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