Guanine nucleotide binding protein involved in muscarinic responses in the pig coronary artery is insensitive to islet-activating protein

Toshiyuki Sasaguri, Masato Hirata, T. Itoh, T. Koga, H. Kuriyama

Research output: Contribution to journalArticle

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Abstract

In an attempt to identify the nature of guanine nucleotide binding protein(s) (G-protein) involved in the acetylcholine (ACh)-induced (muscarinic) response of pig coronary-artery smooth muscle, we studied the effect of ADP-ribosylation of specific membrane protein(s) catalysed by islet-activating protein (IAP; pertussis toxin). The ACh-stimulated and guanine nucleotide-dependent activities of phosphatidylinositol 4,5-bisphosphate (PIP 2 ) phosphodiesterase (PDE), assessed by the production of inositol 1,4,5-trisphosphate (IP 3 ) from exogenously applied PIP 2 , were not modified, in either IAP-treated or non-treated cell homogenates used as the enzyme source. In intact tissues, pretreatment with up to 100 ng of IAP/ml inhibited neither the ACh-induced decrease in the amount of inositol phospholipids nor the increase in the amounts of phosphatidic acid and of inositol phosphates. IAP treatment increased the amount of cyclic AMP accumulated by isoprenaline. These observations suggest that G-protein which couples the muscarinic receptor to PIP 2 -PDE is insensitive to IAP. Such being the case, the nature of this protein(s) probably differs from that required for the regulation of adenylate cyclase activities (N(i) or G(i)).

Original languageEnglish
Pages (from-to)567-574
Number of pages8
JournalBiochemical Journal
Volume239
Issue number3
DOIs
Publication statusPublished - Jan 1 1986

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Guanine Nucleotides
Pertussis Toxin
Cholinergic Agents
Acetylcholine
Coronary Vessels
Carrier Proteins
Swine
Phosphatidylinositols
GTP-Binding Proteins
Phosphatidic Acids
Inositol 1,4,5-Trisphosphate
Inositol Phosphates
Muscarinic Receptors
Isoproterenol
Adenylyl Cyclases
Cyclic AMP
Adenosine Diphosphate
Smooth Muscle
Muscle
Membrane Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Guanine nucleotide binding protein involved in muscarinic responses in the pig coronary artery is insensitive to islet-activating protein. / Sasaguri, Toshiyuki; Hirata, Masato; Itoh, T.; Koga, T.; Kuriyama, H.

In: Biochemical Journal, Vol. 239, No. 3, 01.01.1986, p. 567-574.

Research output: Contribution to journalArticle

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