Guard cell chloroplasts are essential for blue light-dependent stomatal opening in arabidopsis

Noriyuki Suetsugu, Tsuneaki Takami, Yuuta Ebisu, Harutaka Watanabe, Chihoko Iiboshi, Michio Doi, Ken Ichiro Shimazaki

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Blue light (BL) induces stomatal opening through the activation of H+-ATPases with subsequent ion accumulation in guard cells. In most plant species, red light (RL) enhances BL-dependent stomatal opening. This RL effect is attributable to the chloroplasts of guard cell, the only cells in the epidermis possessing this organelle. To clarify the role of chloroplasts in stomatal regulation, we investigated the effects of RL on BL-dependent stomatal opening in isolated epidermis, guard cell protoplasts, and intact leaves of Arabidopsis thaliana. In isolated epidermal tissues and intact leaves, weak BL superimposed on RL enhanced stomatal opening while BL alone was less effective. In guard cell protoplasts, RL enhanced BL-dependent H+-pumping and DCMU, a photosynthetic electron transport inhibitor, eliminated this effect. RL enhanced phosphorylation levels of the H+-ATPase in response to BL, but this RL effect was not suppressed by DCMU. Furthermore, DCMU inhibited both RL-induced and BL-dependent stomatal opening in intact leaves. The photosynthetic rate in leaves correlated positively with BL-dependent stomatal opening in the presence of DCMU. We conclude that guard cell chloroplasts provide ATP and/or reducing equivalents that fuel BL-dependent stomatal opening, and that they indirectly monitor photosynthetic CO2fixation in mesophyll chloroplasts by absorbing PAR in the epidermis.

Original languageEnglish
Article numbere108374
JournalPloS one
Volume9
Issue number9
DOIs
Publication statusPublished - Sep 24 2014

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guard cells
Chloroplasts
blue light
Arabidopsis
red light
chloroplasts
Light
epidermis (plant)
Diuron
H-transporting ATP synthase
protoplasts
leaves
Epidermis
Proton-Translocating ATPases
Protoplasts
mesophyll
electron transfer
organelles
phosphorylation
Arabidopsis thaliana

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Suetsugu, N., Takami, T., Ebisu, Y., Watanabe, H., Iiboshi, C., Doi, M., & Shimazaki, K. I. (2014). Guard cell chloroplasts are essential for blue light-dependent stomatal opening in arabidopsis. PloS one, 9(9), [e108374]. https://doi.org/10.1371/journal.pone.0108374

Guard cell chloroplasts are essential for blue light-dependent stomatal opening in arabidopsis. / Suetsugu, Noriyuki; Takami, Tsuneaki; Ebisu, Yuuta; Watanabe, Harutaka; Iiboshi, Chihoko; Doi, Michio; Shimazaki, Ken Ichiro.

In: PloS one, Vol. 9, No. 9, e108374, 24.09.2014.

Research output: Contribution to journalArticle

Suetsugu, N, Takami, T, Ebisu, Y, Watanabe, H, Iiboshi, C, Doi, M & Shimazaki, KI 2014, 'Guard cell chloroplasts are essential for blue light-dependent stomatal opening in arabidopsis', PloS one, vol. 9, no. 9, e108374. https://doi.org/10.1371/journal.pone.0108374
Suetsugu N, Takami T, Ebisu Y, Watanabe H, Iiboshi C, Doi M et al. Guard cell chloroplasts are essential for blue light-dependent stomatal opening in arabidopsis. PloS one. 2014 Sep 24;9(9). e108374. https://doi.org/10.1371/journal.pone.0108374
Suetsugu, Noriyuki ; Takami, Tsuneaki ; Ebisu, Yuuta ; Watanabe, Harutaka ; Iiboshi, Chihoko ; Doi, Michio ; Shimazaki, Ken Ichiro. / Guard cell chloroplasts are essential for blue light-dependent stomatal opening in arabidopsis. In: PloS one. 2014 ; Vol. 9, No. 9.
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