Guest-binding behavior of peptide nanocapsules self-assembled from viral peptide fragments

Kazunori Matsuura, Kenta Watanabe, Yoshihiro Matsushita, Nobuo Kimizuka

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The binding behavior of guests (dyes and DNA) into peptide nanocapsules formed via self-assembly of a 24-mer β-annulus peptide fragment obtained from the capsid protein of the tomato bushy stunt virus is reported. The pH dependence of the ζ potential of the peptide nanocapsules indicates that the C- and N-termini are directed to the exterior and interior of the nanocapsules, respectively. Equilibrium dialysis experiments with dyes and the peptide nanocapsules at pH 7 showed that the peptide nanocapsules tend to bind anionic dyes. Binding of sodium 8-anilinonaphthalene-1-sulfonate and uranine into the peptide nanocapsules minimally affected the size of the nanocapsules, whereas binding of other anionic dyes resulted in the formation of precipitates. In addition, binding of Thioflavin T to the β-annulus peptide promoted disassembly of the nanocapsules. Complexation of the β-annulus peptide with M13 phage DNA formed a core-shell nanosphere in which the DNA was encapsulated in the peptide assembly.

Original languageEnglish
Pages (from-to)529-534
Number of pages6
JournalPolymer Journal
Volume45
Issue number5
DOIs
Publication statusPublished - May 1 2013

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Nanocapsules
Peptide Fragments
Peptides
Coloring Agents
Dyes
DNA
Bacteriophages
Dialysis
Nanospheres
Capsid Proteins
Complexation
Fluorescein
Viruses
Self assembly
Precipitates
Sodium
Proteins

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics
  • Materials Chemistry

Cite this

Guest-binding behavior of peptide nanocapsules self-assembled from viral peptide fragments. / Matsuura, Kazunori; Watanabe, Kenta; Matsushita, Yoshihiro; Kimizuka, Nobuo.

In: Polymer Journal, Vol. 45, No. 5, 01.05.2013, p. 529-534.

Research output: Contribution to journalArticle

Matsuura, Kazunori ; Watanabe, Kenta ; Matsushita, Yoshihiro ; Kimizuka, Nobuo. / Guest-binding behavior of peptide nanocapsules self-assembled from viral peptide fragments. In: Polymer Journal. 2013 ; Vol. 45, No. 5. pp. 529-534.
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