TY - JOUR
T1 - Guide to the Assessment of Mature Liver Gene Expression in Stem Cell-Derived Hepatocytes
AU - Zabulica, Mihaela
AU - Srinivasan, Raghuraman C.
AU - Vosough, Massoud
AU - Hammarstedt, Christina
AU - Wu, Tingting
AU - Gramignoli, Roberto
AU - Ellis, Ewa
AU - Kannisto, Kristina
AU - Collin De L'Hortet, Alexandra
AU - Takeishi, Kazuki
AU - Soto-Gutierrez, Alejandro
AU - Strom, Stephen C.
N1 - Funding Information:
This research was supported by CIMED, Clinical Innovative Medicine, Ventenskaprådet (Swedish Research Council) European Commission, EU/FP7, HUMAN and the Torsten och Ragnar Söderberg Stiftelse (SCS). This work was supported by grants from NIH, DK099257 and DK117881 to A.S.-G., and by the American Liver Foundation and the Uehara Memorial Foundation to K.T.
Publisher Copyright:
© Mihaela Zabulica et al. 2019; Published by Mary Ann Liebert, Inc. 2019.
PY - 2019/7/15
Y1 - 2019/7/15
N2 - Differentiation of stem cells to hepatocyte-like cells (HLCs) holds great promise for basic research, drug and toxicological investigations, and clinical applications. There are currently no protocols for the production of HLCs from stem cells, such as embryonic stem cells or induced pluripotent stem cells, that produce fully mature hepatocytes with a wide range of mature hepatic functions. This report describes a standard method to assess the maturation of stem cell-derived HLCs with a moderately high-throughput format, by analysing liver gene expression by quantitative RT-qPCR. This method also provides a robust data set of the expression of 62 genes expressed in normal liver, generated from 17 fetal and 25 mature human livers, so that investigators can quickly and easily compare the expression of these genes in their stem cell-derived HLCs with the values obtained in authentic fetal and mature human liver. The simple methods described in this study will provide a quick and accurate assessment of the efficacy of a differentiation protocol and will help guide the optimization of differentiation conditions.
AB - Differentiation of stem cells to hepatocyte-like cells (HLCs) holds great promise for basic research, drug and toxicological investigations, and clinical applications. There are currently no protocols for the production of HLCs from stem cells, such as embryonic stem cells or induced pluripotent stem cells, that produce fully mature hepatocytes with a wide range of mature hepatic functions. This report describes a standard method to assess the maturation of stem cell-derived HLCs with a moderately high-throughput format, by analysing liver gene expression by quantitative RT-qPCR. This method also provides a robust data set of the expression of 62 genes expressed in normal liver, generated from 17 fetal and 25 mature human livers, so that investigators can quickly and easily compare the expression of these genes in their stem cell-derived HLCs with the values obtained in authentic fetal and mature human liver. The simple methods described in this study will provide a quick and accurate assessment of the efficacy of a differentiation protocol and will help guide the optimization of differentiation conditions.
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U2 - 10.1089/scd.2019.0064
DO - 10.1089/scd.2019.0064
M3 - Article
C2 - 31122128
AN - SCOPUS:85069186888
SN - 1547-3287
VL - 28
SP - 907
EP - 919
JO - Stem Cells and Development
JF - Stem Cells and Development
IS - 14
ER -