Gurmarin sensitivity of sweet taste responses is associated with co-expression patterns of T1r2, T1r3, and gustducin

Noriatsu Shigemura, Kazuko Nakao, Toshiaki Yasuo, Yoshihiro Murata, Keiko Nakano, Akihiko Nakashima, Hideo Katsukawa, Noritaka Sako, Yuzo Ninomiya

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Abstract

Gurmarin (Gur) is a peptide that selectively suppresses sweet taste responses in rodents. The inhibitory effect of Gur differs among tongue regions and mouse strains. Recent studies demonstrated that co-expression levels of genes controlling sweet receptors (T1r2/T1r3 heterodimer) versus Gα-protein, gustducin, are much lower in Gur-insensitive posterior circumvallate papillae than in Gur-sensitive anterior fungiform papillae. Here, we investigated the potential link of Gur-sensitivity with the co-expression for T1r2/T1r3 receptors and gustducin by comparing those of taste tissues of Gur-sensitive (B6, dpa congenic strains) and Gur-weakly-sensitive (BALB) strains. The results indicated that co-expression ratios among T1r2, T1r3, and gustducin in the fungiform papillae were significantly lower in Gur-weakly-sensitive BALB mice than in Gur-sensitive B6 and dpa congenic mice. This linkage between Gur-sensitivity and co-expression for T1r2/T1r3 receptors versus gustducin suggests that gustducin may be a key molecule involved in the pathway for Gur-sensitive sweet responses.

Original languageEnglish
Pages (from-to)356-363
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume367
Issue number2
DOIs
Publication statusPublished - Mar 7 2008

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gustducin
Gymnema sylvestre Gurmarin protein
Congenic Mice
GTP-Binding Proteins
Tongue
Rodentia
Genes
Tissue
Gene Expression
Peptides
Molecules

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

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Gurmarin sensitivity of sweet taste responses is associated with co-expression patterns of T1r2, T1r3, and gustducin. / Shigemura, Noriatsu; Nakao, Kazuko; Yasuo, Toshiaki; Murata, Yoshihiro; Nakano, Keiko; Nakashima, Akihiko; Katsukawa, Hideo; Sako, Noritaka; Ninomiya, Yuzo.

In: Biochemical and Biophysical Research Communications, Vol. 367, No. 2, 07.03.2008, p. 356-363.

Research output: Contribution to journalArticle

Shigemura, Noriatsu ; Nakao, Kazuko ; Yasuo, Toshiaki ; Murata, Yoshihiro ; Nakano, Keiko ; Nakashima, Akihiko ; Katsukawa, Hideo ; Sako, Noritaka ; Ninomiya, Yuzo. / Gurmarin sensitivity of sweet taste responses is associated with co-expression patterns of T1r2, T1r3, and gustducin. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 367, No. 2. pp. 356-363.
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