Gustducin is expressed in the taste buds of the chicken

Ken Ichi Kudo, Kei Ichi Wakamatsu, Shotaro Nishimura, Shoji Tabata

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We investigated the expression of gustducin in chicken taste buds using molecular biological, biochemical and immunohistochemical techniques. Expression of a gustducin-like sequence was detected by RT-PCR in the tissues containing taste buds, and corresponded to the predicted gustducin gene in the chicken. Expression of this sequence was not detected in the brain, heart, liver, pancreas, intestine, kidney and testis of the chicken. The expressed sequence had a high specificity for oral tissues that contained taste buds. These results suggest that the detected sequence was the chicken gustducin gene. Next, we generated a polyclonal antiserum against the chicken gustducin protein to observe its localization in the oral tissues. The results revealed that the chicken gustducin was specifically expressed in the taste buds. It is suggested that the chicken has a gustatory system mediated by gustducin, and chicken gustducin is a reliable marker for taste buds or taste cells. This is the first molecular biological, biochemical and immunohistochemical demonstration of the presence of gustducin in the chicken.

Original languageEnglish
Pages (from-to)666-672
Number of pages7
JournalAnimal Science Journal
Volume81
Issue number6
DOIs
Publication statusPublished - Dec 1 2010

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Taste Buds
palps
Chickens
chickens
mouth
gustducin
Organ Specificity
pancreas
Genes
Intestines
antiserum
Testis
Immune Sera
Pancreas
testes
intestines
genes
reverse transcriptase polymerase chain reaction
heart
kidneys

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

Cite this

Gustducin is expressed in the taste buds of the chicken. / Kudo, Ken Ichi; Wakamatsu, Kei Ichi; Nishimura, Shotaro; Tabata, Shoji.

In: Animal Science Journal, Vol. 81, No. 6, 01.12.2010, p. 666-672.

Research output: Contribution to journalArticle

Kudo, Ken Ichi ; Wakamatsu, Kei Ichi ; Nishimura, Shotaro ; Tabata, Shoji. / Gustducin is expressed in the taste buds of the chicken. In: Animal Science Journal. 2010 ; Vol. 81, No. 6. pp. 666-672.
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