Gut dysbiosis associated with hepatitis C virus infection

Takako Inoue, Jiro Nakayama, Kei Moriya, Hideto Kawaratani, Rie Momoda, Kiyoaki Ito, Etsuko Iio, Shunsuke Nojiri, Kei Fujiwara, Masashi Yoneda, Hitoshi Yoshiji, Yasuhito Tanaka

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background. Little is known about the effect of hepatitis C virus (HCV) infection on gut microbiota and the relationship between alteration of gut microbiota and chronic hepatitis C (CHC) progression. We performed a comparative study of gut microbiota composition between CHC patients and healthy individuals. Methods. Fecal samples from 166 CHC patients were compared with those from 23 healthy individuals; the gut microbiota community was analyzed using 16S ribosomal RNA gene sequencing. CHC patients were diagnosed with persistently normal serum alanine aminotransferase without evidence of liver cirrhosis (LC) (PNALT, n = 18), chronic hepatitis (CH, n = 84), LC (n = 40), and hepatocellular carcinoma in LC (n = 24). Results. Compared with healthy individuals, bacterial diversity was lower in persons with HCV infection, with a decrease in the order Clostridiales and an increase in Streptococcus and Lactobacillus. Microbiota dysbiosis already appeared in the PNALT stage with the transient increase in Bacteroides and Enterobacteriaceae. Predicted metagenomics of microbial communities showed an increase in the urease gene mainly encoded by viridans streptococci during CHC progression, consistent with a significantly higher fecal pH in CH and LC patients than in healthy individuals or those in the PNALT stage. Conclusions. HCV infection is associated with gut dysbiosis, even in patients with mild liver disease. Additionally, overgrowth of viridans streptococci can account for hyperammonemia in CH and LC. Further studies would help to propose a novel treatment strategy because the gut microbiome can be therapeutically altered, potentially reducing the complications of chronic liver disease.

Original languageEnglish
Pages (from-to)869-877
Number of pages9
JournalClinical Infectious Diseases
Volume67
Issue number6
DOIs
Publication statusPublished - Jan 1 2018

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Dysbiosis
Chronic Hepatitis C
Virus Diseases
Hepacivirus
Liver Cirrhosis
Viridans Streptococci
Liver Diseases
16S Ribosomal RNA
RNA Sequence Analysis
Hyperammonemia
Metagenomics
Bacteroides
Urease
Microbiota
Lactobacillus
Enterobacteriaceae
Chronic Hepatitis
Streptococcus
Alanine Transaminase
rRNA Genes

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Inoue, T., Nakayama, J., Moriya, K., Kawaratani, H., Momoda, R., Ito, K., ... Tanaka, Y. (2018). Gut dysbiosis associated with hepatitis C virus infection. Clinical Infectious Diseases, 67(6), 869-877. https://doi.org/10.1093/cid/ciy205

Gut dysbiosis associated with hepatitis C virus infection. / Inoue, Takako; Nakayama, Jiro; Moriya, Kei; Kawaratani, Hideto; Momoda, Rie; Ito, Kiyoaki; Iio, Etsuko; Nojiri, Shunsuke; Fujiwara, Kei; Yoneda, Masashi; Yoshiji, Hitoshi; Tanaka, Yasuhito.

In: Clinical Infectious Diseases, Vol. 67, No. 6, 01.01.2018, p. 869-877.

Research output: Contribution to journalArticle

Inoue, T, Nakayama, J, Moriya, K, Kawaratani, H, Momoda, R, Ito, K, Iio, E, Nojiri, S, Fujiwara, K, Yoneda, M, Yoshiji, H & Tanaka, Y 2018, 'Gut dysbiosis associated with hepatitis C virus infection', Clinical Infectious Diseases, vol. 67, no. 6, pp. 869-877. https://doi.org/10.1093/cid/ciy205
Inoue, Takako ; Nakayama, Jiro ; Moriya, Kei ; Kawaratani, Hideto ; Momoda, Rie ; Ito, Kiyoaki ; Iio, Etsuko ; Nojiri, Shunsuke ; Fujiwara, Kei ; Yoneda, Masashi ; Yoshiji, Hitoshi ; Tanaka, Yasuhito. / Gut dysbiosis associated with hepatitis C virus infection. In: Clinical Infectious Diseases. 2018 ; Vol. 67, No. 6. pp. 869-877.
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