Gut microbiota of Pheretima heteropoda and allolobophorajaponica fed under the same laboratory conditions

Kazunori Nakamura, Kenji Sakai

Research output: Contribution to journalArticle

Abstract

Our previous study reported that the gut microbiota of Megascolecidae was distinguishable from that of Lumbricidae. In this study, two earthworms, Pheretima heteropoda (Megascolecidae) and Allolobophora japonica (Lumbricidae), were fed under the same in vitro conditions to investigate the effect of feed on gut microbiota. Their gut microbiota was analyzed by denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments. Most of the dominant bands were shared between both earthworm species, but some were specific to one species. These banding patterns corresponded to in situ earthworms, i.e. they were directly analyzed after collection from the field. The predominant bacterium found in P. heteropoda gut samples was Bacillus longiquaesitum (AM747042)-related, whereas the predominant bacteria found in A. japonica gut samples were bacterium TG141 (AB308367)-related and Rhizobium sp. BBCT66 (DQ337551)-related. These results indicated that the major bacterial species were not influenced by the surrounding environment, including the food source, even though a large portion of the bacterial population was opportunistic.

Original languageEnglish
Pages (from-to)71-74
Number of pages4
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume63
Issue number1
Publication statusPublished - Feb 1 2018

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Heteropoda
Pheretima
Oligochaeta
intestinal microorganisms
earthworms
Megascolecidae
Lumbricidae
Bacteria
bacteria
Allolobophora
digestive system
Denaturing Gradient Gel Electrophoresis
Rhizobium
Bacillus (bacteria)
denaturing gradient gel electrophoresis
rRNA Genes
Bacillus
ribosomal RNA
sampling
Food

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Gut microbiota of Pheretima heteropoda and allolobophorajaponica fed under the same laboratory conditions. / Nakamura, Kazunori; Sakai, Kenji.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 63, No. 1, 01.02.2018, p. 71-74.

Research output: Contribution to journalArticle

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