Gymnosperms have increased capacity for electron leakage to oxygen (Mehler and PTOX reactions) in photosynthesis compared with angiosperms

Masayoshi Shirao, Shu Kuroki, Kaoru Kaneko, Yuriko Kinjo, Michito Tsuyama, Britta Förster, Shunichi Takahashi, Murray R. Badger

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Oxygen plays an important role in photosynthesis by participating in a number of O2-consuming reactions. O2 inhibits CO 2 fixation by stimulating photorespiration, thus reducing plant production. O2 interacts with photosynthetic electron transport in the chloroplasts' thylakoids in two main ways: by accepting electrons from PSI (Mehler reaction); and by accepting electrons from reduced plastoquinone (PQ) mediated by the plastid terminal oxidase (PTOX). In this study, we show, using 101 plant species, that there is a difference in the potential for photosynthetic electron flow to O2 between angiosperms and gymnosperms. We found, from measurements of Chl fluorescence and leaf absorbance at 830 nm, (i) that electron outflow from PSII, as determined by decay kinetics of Chl fluorescence after application of a saturating light pulse, is more rapid in gymnosperms than in angiosperms; (ii) that the reaction center Chl of PSI (P700) is rapidly and highly oxidized in gymnosperms during induction of photosynthesis; and (iii) that these differences are dependent on oxygen. Finally, rates of O2 uptake measured by mass spectrometry in the absence of photorespiration were significantly promoted by illumination in dark-adapted leaves of gymnosperms, but not in those of angiosperms. The light-stimulated O2 uptake was around 10% of the maximum O 2 evolution in gymnosperms and 1% in angiosperms. These results suggest that gymnosperms have increased capacity for electron leakage to oxygen in photosynthesis compared with angiosperms. The involvement of the Mehler reaction and PTOX in the electron flow to O2 is discussed.

Original languageEnglish
Pages (from-to)1152-1163
Number of pages12
JournalPlant and Cell Physiology
Volume54
Issue number7
DOIs
Publication statusPublished - Jul 1 2013

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Gymnosperms
Angiosperms
Plastids
Photosynthesis
plastids
Angiospermae
Oxidoreductases
electrons
photosynthesis
Electrons
Oxygen
oxygen
photorespiration
Fluorescence
Plastoquinone
fluorescence
uptake mechanisms
Light
Thylakoids
Chloroplasts

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Physiology
  • Plant Science
  • Cell Biology

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Gymnosperms have increased capacity for electron leakage to oxygen (Mehler and PTOX reactions) in photosynthesis compared with angiosperms. / Shirao, Masayoshi; Kuroki, Shu; Kaneko, Kaoru; Kinjo, Yuriko; Tsuyama, Michito; Förster, Britta; Takahashi, Shunichi; Badger, Murray R.

In: Plant and Cell Physiology, Vol. 54, No. 7, 01.07.2013, p. 1152-1163.

Research output: Contribution to journalArticle

Shirao, Masayoshi ; Kuroki, Shu ; Kaneko, Kaoru ; Kinjo, Yuriko ; Tsuyama, Michito ; Förster, Britta ; Takahashi, Shunichi ; Badger, Murray R. / Gymnosperms have increased capacity for electron leakage to oxygen (Mehler and PTOX reactions) in photosynthesis compared with angiosperms. In: Plant and Cell Physiology. 2013 ; Vol. 54, No. 7. pp. 1152-1163.
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