H-, K-, and N-ras gene mutation in atypical fibroxanthoma and malignant fibrous histiocytoma

Akio Sakamoto, Yoshinao Oda, Eijun Itakura, Yumi Oshiro, Sadafumi Tamiya, Yumi Honda, Akira Ishihara, Yukihide Iwamoto, Masazumi Tsuneyoshi

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Atypical fibroxanthoma (AFX) which is histologically similar to malignant fibrous histiocytoma (MFH), occurs in the sun-exposed skin. The presence of mutations at codons 12 and 13 of the H- and K-ras genes and in exons 1 and 2, which include codons 12, 13, and 61, of the N-ras gene was studied in 8 cases of AFX and 8 cases of storiform-pleomorphic-type MFH using polymerase chain reaction (PCR)-restriction fragment length polymorphism and PCR-single-conformation polymorphism. Two of the 8 cases of MFH showed ras mutations in the H-ras gene at codon 12 (GGC-AGC) and in the K-ras gene at codon 13 (GGC-GAC). H- and K-ras gene mutations were not seen in any of the cases of AFX (0 of 8). N-ras gene mutation was not detected in either the AFX (0 of 8) or MFH (0 of 8) cases. In conclusion, although the number of cases in this study was small, H-and K-ras genes were present in some of the MFH cases and accordingly may play an important role in the pathogenesis of MFH. In addition, the finding that H-, K-, and N-ras gene mutations are not present in AFX may indicate why AFX has a more favorable behavior than MFH.

Original languageEnglish
Pages (from-to)1225-1231
Number of pages7
JournalHuman Pathology
Volume32
Issue number11
DOIs
Publication statusPublished - Jan 1 2001

Fingerprint

Malignant Fibrous Histiocytoma
ras Genes
Mutation
Codon
Polymerase Chain Reaction
Solar System
Restriction Fragment Length Polymorphisms
Exons

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Sakamoto, A., Oda, Y., Itakura, E., Oshiro, Y., Tamiya, S., Honda, Y., ... Tsuneyoshi, M. (2001). H-, K-, and N-ras gene mutation in atypical fibroxanthoma and malignant fibrous histiocytoma. Human Pathology, 32(11), 1225-1231. https://doi.org/10.1053/hupa.2001.28953

H-, K-, and N-ras gene mutation in atypical fibroxanthoma and malignant fibrous histiocytoma. / Sakamoto, Akio; Oda, Yoshinao; Itakura, Eijun; Oshiro, Yumi; Tamiya, Sadafumi; Honda, Yumi; Ishihara, Akira; Iwamoto, Yukihide; Tsuneyoshi, Masazumi.

In: Human Pathology, Vol. 32, No. 11, 01.01.2001, p. 1225-1231.

Research output: Contribution to journalArticle

Sakamoto, A, Oda, Y, Itakura, E, Oshiro, Y, Tamiya, S, Honda, Y, Ishihara, A, Iwamoto, Y & Tsuneyoshi, M 2001, 'H-, K-, and N-ras gene mutation in atypical fibroxanthoma and malignant fibrous histiocytoma', Human Pathology, vol. 32, no. 11, pp. 1225-1231. https://doi.org/10.1053/hupa.2001.28953
Sakamoto, Akio ; Oda, Yoshinao ; Itakura, Eijun ; Oshiro, Yumi ; Tamiya, Sadafumi ; Honda, Yumi ; Ishihara, Akira ; Iwamoto, Yukihide ; Tsuneyoshi, Masazumi. / H-, K-, and N-ras gene mutation in atypical fibroxanthoma and malignant fibrous histiocytoma. In: Human Pathology. 2001 ; Vol. 32, No. 11. pp. 1225-1231.
@article{c7410959dbd848f6a6b6e32bbf1ebd11,
title = "H-, K-, and N-ras gene mutation in atypical fibroxanthoma and malignant fibrous histiocytoma",
abstract = "Atypical fibroxanthoma (AFX) which is histologically similar to malignant fibrous histiocytoma (MFH), occurs in the sun-exposed skin. The presence of mutations at codons 12 and 13 of the H- and K-ras genes and in exons 1 and 2, which include codons 12, 13, and 61, of the N-ras gene was studied in 8 cases of AFX and 8 cases of storiform-pleomorphic-type MFH using polymerase chain reaction (PCR)-restriction fragment length polymorphism and PCR-single-conformation polymorphism. Two of the 8 cases of MFH showed ras mutations in the H-ras gene at codon 12 (GGC-AGC) and in the K-ras gene at codon 13 (GGC-GAC). H- and K-ras gene mutations were not seen in any of the cases of AFX (0 of 8). N-ras gene mutation was not detected in either the AFX (0 of 8) or MFH (0 of 8) cases. In conclusion, although the number of cases in this study was small, H-and K-ras genes were present in some of the MFH cases and accordingly may play an important role in the pathogenesis of MFH. In addition, the finding that H-, K-, and N-ras gene mutations are not present in AFX may indicate why AFX has a more favorable behavior than MFH.",
author = "Akio Sakamoto and Yoshinao Oda and Eijun Itakura and Yumi Oshiro and Sadafumi Tamiya and Yumi Honda and Akira Ishihara and Yukihide Iwamoto and Masazumi Tsuneyoshi",
year = "2001",
month = "1",
day = "1",
doi = "10.1053/hupa.2001.28953",
language = "English",
volume = "32",
pages = "1225--1231",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders Ltd",
number = "11",

}

TY - JOUR

T1 - H-, K-, and N-ras gene mutation in atypical fibroxanthoma and malignant fibrous histiocytoma

AU - Sakamoto, Akio

AU - Oda, Yoshinao

AU - Itakura, Eijun

AU - Oshiro, Yumi

AU - Tamiya, Sadafumi

AU - Honda, Yumi

AU - Ishihara, Akira

AU - Iwamoto, Yukihide

AU - Tsuneyoshi, Masazumi

PY - 2001/1/1

Y1 - 2001/1/1

N2 - Atypical fibroxanthoma (AFX) which is histologically similar to malignant fibrous histiocytoma (MFH), occurs in the sun-exposed skin. The presence of mutations at codons 12 and 13 of the H- and K-ras genes and in exons 1 and 2, which include codons 12, 13, and 61, of the N-ras gene was studied in 8 cases of AFX and 8 cases of storiform-pleomorphic-type MFH using polymerase chain reaction (PCR)-restriction fragment length polymorphism and PCR-single-conformation polymorphism. Two of the 8 cases of MFH showed ras mutations in the H-ras gene at codon 12 (GGC-AGC) and in the K-ras gene at codon 13 (GGC-GAC). H- and K-ras gene mutations were not seen in any of the cases of AFX (0 of 8). N-ras gene mutation was not detected in either the AFX (0 of 8) or MFH (0 of 8) cases. In conclusion, although the number of cases in this study was small, H-and K-ras genes were present in some of the MFH cases and accordingly may play an important role in the pathogenesis of MFH. In addition, the finding that H-, K-, and N-ras gene mutations are not present in AFX may indicate why AFX has a more favorable behavior than MFH.

AB - Atypical fibroxanthoma (AFX) which is histologically similar to malignant fibrous histiocytoma (MFH), occurs in the sun-exposed skin. The presence of mutations at codons 12 and 13 of the H- and K-ras genes and in exons 1 and 2, which include codons 12, 13, and 61, of the N-ras gene was studied in 8 cases of AFX and 8 cases of storiform-pleomorphic-type MFH using polymerase chain reaction (PCR)-restriction fragment length polymorphism and PCR-single-conformation polymorphism. Two of the 8 cases of MFH showed ras mutations in the H-ras gene at codon 12 (GGC-AGC) and in the K-ras gene at codon 13 (GGC-GAC). H- and K-ras gene mutations were not seen in any of the cases of AFX (0 of 8). N-ras gene mutation was not detected in either the AFX (0 of 8) or MFH (0 of 8) cases. In conclusion, although the number of cases in this study was small, H-and K-ras genes were present in some of the MFH cases and accordingly may play an important role in the pathogenesis of MFH. In addition, the finding that H-, K-, and N-ras gene mutations are not present in AFX may indicate why AFX has a more favorable behavior than MFH.

UR - http://www.scopus.com/inward/record.url?scp=0035191597&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035191597&partnerID=8YFLogxK

U2 - 10.1053/hupa.2001.28953

DO - 10.1053/hupa.2001.28953

M3 - Article

C2 - 11727262

AN - SCOPUS:0035191597

VL - 32

SP - 1225

EP - 1231

JO - Human Pathology

JF - Human Pathology

SN - 0046-8177

IS - 11

ER -