H-ras oncogene mutation in dedifferentiated liposarcoma

Polymerase chain reaction-restriction fragment length polymorphism analysis

A. Sakamoto, Yoshinao Oda, T. Adachi, S. Tamiya, S. Matsuda, K. Tanaka, Y. Iwamoto, M. Tsuneyoshi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Point mutations of the ras gene family (K-ras, H-ras, and N-ras) are thought to be involved in the development of a variety of human tumors. Dedifferentiated liposarcoma is characterized by the coexistence of well-differentiated (WD) and high-grade anaplastic (HG) components. The presence of point mutations at codons 12 and 13 of the H-ras gene was studied in 34 liposarcomas, comprising 15 welldifferentiated liposarcomas and 19 dedifferentiated liposarcomas, and in 8 storiform-pleomorphic type malignant fibrous histiocytomas (MFHs) using polymerase chain reaction-restriction fragment length polymorphism and direct sequencing analysis. The 2 components of dedifferentiated liposarcoma were analyzed independently. H-ras mutations were seen only in dedifferentiated liposarcomas (4/19 [21%]), 1 in WD components and 3 in HG components. The mutation was not seen in any of 15 cases of well-differentiated liposarcoma. MFHs showed an H-ras mutation in 1 (12%) of 8 cases. Our results seem to suggest that the H-ras mutation is a relatively uncommon event in dedifferentiated liposarcoma, which may demonstrate an epiphenomenon of dedifferentiation in dedifferentiated liposarcoma.

Original languageEnglish
Pages (from-to)235-242
Number of pages8
JournalAmerican Journal of Clinical Pathology
Volume115
Issue number2
DOIs
Publication statusPublished - Feb 24 2001

Fingerprint

Liposarcoma
ras Genes
Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction
Mutation
Malignant Fibrous Histiocytoma
Point Mutation
Codon

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

H-ras oncogene mutation in dedifferentiated liposarcoma : Polymerase chain reaction-restriction fragment length polymorphism analysis. / Sakamoto, A.; Oda, Yoshinao; Adachi, T.; Tamiya, S.; Matsuda, S.; Tanaka, K.; Iwamoto, Y.; Tsuneyoshi, M.

In: American Journal of Clinical Pathology, Vol. 115, No. 2, 24.02.2001, p. 235-242.

Research output: Contribution to journalArticle

Sakamoto, A. ; Oda, Yoshinao ; Adachi, T. ; Tamiya, S. ; Matsuda, S. ; Tanaka, K. ; Iwamoto, Y. ; Tsuneyoshi, M. / H-ras oncogene mutation in dedifferentiated liposarcoma : Polymerase chain reaction-restriction fragment length polymorphism analysis. In: American Journal of Clinical Pathology. 2001 ; Vol. 115, No. 2. pp. 235-242.
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