H-ras oncogene mutation in dedifferentiated liposarcoma: Polymerase chain reaction-restriction fragment length polymorphism analysis

A. Sakamoto, Y. Oda, T. Adachi, S. Tamiya, S. Matsuda, K. Tanaka, Y. Iwamoto, M. Tsuneyoshi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Point mutations of the ras gene family (K-ras, H-ras, and N-ras) are thought to be involved in the development of a variety of human tumors. Dedifferentiated liposarcoma is characterized by the coexistence of well-differentiated (WD) and high-grade anaplastic (HG) components. The presence of point mutations at codons 12 and 13 of the H-ras gene was studied in 34 liposarcomas, comprising 15 welldifferentiated liposarcomas and 19 dedifferentiated liposarcomas, and in 8 storiform-pleomorphic type malignant fibrous histiocytomas (MFHs) using polymerase chain reaction-restriction fragment length polymorphism and direct sequencing analysis. The 2 components of dedifferentiated liposarcoma were analyzed independently. H-ras mutations were seen only in dedifferentiated liposarcomas (4/19 [21%]), 1 in WD components and 3 in HG components. The mutation was not seen in any of 15 cases of well-differentiated liposarcoma. MFHs showed an H-ras mutation in 1 (12%) of 8 cases. Our results seem to suggest that the H-ras mutation is a relatively uncommon event in dedifferentiated liposarcoma, which may demonstrate an epiphenomenon of dedifferentiation in dedifferentiated liposarcoma.

Original languageEnglish
Pages (from-to)235-242
Number of pages8
JournalAmerican Journal of Clinical Pathology
Volume115
Issue number2
DOIs
Publication statusPublished - 2001

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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