H2-M3-restricted CD8+ T cells induced by peptide-pulsed dendritic cells confer protection against Mycobacterium tuberculosis

Takehiko Doi, Hisakata Yamada, Toshiki Yajima, Worawidh Wajjwalku, Toshiro Hara, Yasunobu Yoshikai

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19 Citations (Scopus)

Abstract

One of the oligopolymorphic MHC class Ib molecules, H2-M3, presents N-formylated peptides derived from bacteria. In this study, we tested the ability of an H2-M3-binding pepiide, TB2, to induce protection in C57BL/6 mice against Mycobacterium tuberculosis. Immunization with bone marrow-derived dendritic cell (BMDC) pulsed with TB2 or a MI-IC class la-binding peptide, MDPT64190-198 elicited an expansion of Ag-specific CD8+ T cells in the spleen and the lung. The number of TB2-specific CD8+ T cells reached a peak on day 6, contracted with kinetics similar to MPT64 190-198-specific CD8+ T cells and was maintained at an appreciable level for at least 60 days. The TB2-specific CD8+ T cells produced less effector cytokines but have stronger cytotoxic activity than MPT64190-198-specific CD8+ T cells. Mice immunized with TB2-pulsed BMDC as well as those with MPT64190-198-pulsed BMDC showed significant protection against an intratracheal challenge with M. tuberculosis H37Rv. However, histopathology of the lung in mice immunized with TB2-pulsed BMDC was different from mice immunized with MPT64190-198-specific BMDC. Our results suggest that immunization with BMDC pulsed with MHC class 1b-restricted peptides would be a useful vaccination strategy against M. tuberculosis.

Original languageEnglish
Pages (from-to)3806-3813
Number of pages8
JournalJournal of Immunology
Volume178
Issue number6
DOIs
Publication statusPublished - Mar 15 2007

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All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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