Half‐Life of Histone H4 mRNA in Xenopus laevis Embryonic Cells at Different Stages

(histone H4 mRNA/Northern blotting/Xenopus embryonic cells/ half‐life/actinomycin D)

YASUO ATSUCHI, Kosuke Tashiro, YUCHANG FU, KOICHIRO SHIOKAWA

Research output: Contribution to journalArticle

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Abstract

By Northern blotting methods we have recently found that the relative amount of histone H4 mRNA per Xenopus embryo is constant during cleavage, doubles during the gastrula stage, then decreases at the neurula stage to the level slightly lower than that of the cleavage‐embryo. At each developmental stage, the level of mRNA will depend on three parameters: amount of maternal mRNA remaining, rate of the synthesis of the mRNA and rate of the decay of the mRNA. In the present experiment, we compared rates of the decay of H4 mRNA in Xenopus embryonic cells at different stages by pulse‐chase experiments with actinomycin D as an inhibitor of transcription. Under the conditions used, the half‐life of H4 mRNA was estimated to be 90, 80, and 100 min, for the late blastula, late gastrula and neurula stages, respectively. The decay of H4 mRNA with a half‐life of 90 min at the late blastula stage predicts exhaustion of most of maternal H4 mRNA by the early gastruia stage. The slightly longer half‐life of H4 mRNA in neurula than in late gastrula cells suggests that H4 mRNA is more stable in neurula cells than in late gastrula cells, and therefore, the large decrease in the level of H4 mRNA observed at the neurula stage does not depend on the increase in the turnover‐rate of H4 mRNA. Probably, neurula cells synthesize less H4 mRNA than late gastrula cells but utilize it for longer time.

Original languageEnglish
Pages (from-to)239-248
Number of pages10
JournalDevelopment, Growth & Differentiation
Volume29
Issue number3
DOIs
Publication statusPublished - Jan 1 1987

Fingerprint

Xenopus laevis
Dactinomycin
Xenopus
Northern Blotting
Histones
Half-Life
Gastrula
Messenger RNA
RNA Stability
Stored Messenger RNA
Blastula
Embryonic Structures

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

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title = "Half‐Life of Histone H4 mRNA in Xenopus laevis Embryonic Cells at Different Stages: (histone H4 mRNA/Northern blotting/Xenopus embryonic cells/ half‐life/actinomycin D)",
abstract = "By Northern blotting methods we have recently found that the relative amount of histone H4 mRNA per Xenopus embryo is constant during cleavage, doubles during the gastrula stage, then decreases at the neurula stage to the level slightly lower than that of the cleavage‐embryo. At each developmental stage, the level of mRNA will depend on three parameters: amount of maternal mRNA remaining, rate of the synthesis of the mRNA and rate of the decay of the mRNA. In the present experiment, we compared rates of the decay of H4 mRNA in Xenopus embryonic cells at different stages by pulse‐chase experiments with actinomycin D as an inhibitor of transcription. Under the conditions used, the half‐life of H4 mRNA was estimated to be 90, 80, and 100 min, for the late blastula, late gastrula and neurula stages, respectively. The decay of H4 mRNA with a half‐life of 90 min at the late blastula stage predicts exhaustion of most of maternal H4 mRNA by the early gastruia stage. The slightly longer half‐life of H4 mRNA in neurula than in late gastrula cells suggests that H4 mRNA is more stable in neurula cells than in late gastrula cells, and therefore, the large decrease in the level of H4 mRNA observed at the neurula stage does not depend on the increase in the turnover‐rate of H4 mRNA. Probably, neurula cells synthesize less H4 mRNA than late gastrula cells but utilize it for longer time.",
author = "YASUO ATSUCHI and Kosuke Tashiro and YUCHANG FU and KOICHIRO SHIOKAWA",
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AU - ATSUCHI, YASUO

AU - Tashiro, Kosuke

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AU - SHIOKAWA, KOICHIRO

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