Harmful effects of radicals generated in polluted dew on the needles of Japanese Red pine (Pinus densiflora)

Atsushi Kume, Takemitsu Arakaki, Naoko Tsuboi, Masayo Suzuki, Daiki Kuramoto, Kaneyuki Nakane, Hiroshi Sakugawa

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Abstract

The effects of free radicals, ·OH and ·NO, generated in polluted dew water on needles of Pinus densiflora (Japanese Red pine) were investigated. ·OH-generating solutions (HOOH with Fe(III) and oxalate ion; ·OH treatment) and ·OH- and ·NO-generating solutions (NO2-; ·OH/·NO treatment) were regulated at 25, 50 and 100 μmol and pH 4.4. HOOH only (HOOH treatment) was used as a control solution. Solutions were applied as a mist to the needle surface of P. densiflora seedlings before dawn twice a week for 3 months. Within a month, net photosynthesis at near saturating irradiance (Pn) and stomatal conductance (gl) of ·OH-treated needles decreased with increasing solution concentration. The HOOH treatment had no effects on any of the measured parameters. Therefore, ·OH in the artificial dews caused the decreases in Pn and gl. In ·OH/·NO-treated needles, gl increased during the experiment, but Pn was unchanged. In all experiments, the characteristics of PSII were not significantly altered. Free radicals in polluted dew water have harmful effects on the photosynthesis of P. densiflora and compound effects of ·OH and ·NO are different.

Original languageEnglish
Pages (from-to)53-58
Number of pages6
JournalNew Phytologist
Volume152
Issue number1
DOIs
Publication statusPublished - Oct 20 2001

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All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

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