Hda, a novel DnaA-related protein, regulates the replication cycle in Escherichia coli

Jun Ichi Kato, Tsutomu Katayama

Research output: Contribution to journalArticle

195 Citations (Scopus)

Abstract

The bacterial DnaA protein binds to the chromosomal origin of replication to trigger a series of initiation reactions, which leads to the loading of DNA polymerase III. In Escherichia coli, once this polymerase initiates DNA synthesis, ATP bound to DnaA is efficiently hydrolyzed to yield the ADP-bound inactivated form. This negative regulation of DnaA, which occurs through interaction with the β-subunit sliding clamp configuration of the polymerase, functions in the temporal blocking of re-initiation. Here we show that the novel DnaA-related protein, Hda, from E. coli is essential for this regulatory inactivation of DnaA in vitro and in vivo. Our results indicate that the hda gene is required to prevent over-initiation of chromosomal replication and for cell viability. Hda belongs to the chaperone-like ATPase family, AAA+, as do DnaA and certain eukaryotic proteins essential for the initiation of DNA replication. We propose that the once-percell-cycle rule of replication depends on the timely interaction of AAA+ proteins that comprise the apparatus regulating the activity of the initiator of replication.

Original languageEnglish
Pages (from-to)4253-4262
Number of pages10
JournalEMBO Journal
Volume20
Issue number15
DOIs
Publication statusPublished - Aug 1 2001

Fingerprint

Escherichia coli
DNA Polymerase III
Replication Origin
Bacterial Proteins
Clamping devices
DNA-Directed DNA Polymerase
DNA Replication
Adenosine Diphosphate
Adenosine Triphosphatases
Cell Survival
Proteins
Genes
Adenosine Triphosphate
Cells
DNA
E coli Hda protein
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Hda, a novel DnaA-related protein, regulates the replication cycle in Escherichia coli. / Kato, Jun Ichi; Katayama, Tsutomu.

In: EMBO Journal, Vol. 20, No. 15, 01.08.2001, p. 4253-4262.

Research output: Contribution to journalArticle

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