Head and neck cancer antigens recognized by the humoral immune system

Mikio Monji, Satoru Senju, Tetsuya Nakatsura, Kazuhiro Yamada, Motohiro Sawatsubashi, Akira Inokuchi, Yasuharu Nishimura

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Head and neck cancer in advanced stages are difficult to treat. Therefore development of new treatment modalities and preventive measures are required. We now report the identification of human head and neck cancer antigens recognized by the humoral immune system. We used the serological analysis of recombinant cDNA expression libraries (SEREX) approach. cDNA libraries from cell lines of squamous cell carcinoma of head and neck (SCCHN) and a normal testicle tissue were screened using sera from six allogeneic SCCHN patients. Total 28 positive clones belonging to 19 different genes were identified including 12 known genes and 7 unknown ones. Expression analysis on 13 normal tissues and 13 cancer tissues using reverse transcription-PCR (RT-PCR) revealed eight ubiquitously expressed genes nine of which were expressed preferentially in cancer tissues and two cancer/testis antigens. These antigens we defined may be pertinent candidate antigens for future cancer-diagnosis and related immunotherapy.

Original languageEnglish
Pages (from-to)734-741
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume294
Issue number3
DOIs
Publication statusPublished - Jan 1 2002

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Monji, M., Senju, S., Nakatsura, T., Yamada, K., Sawatsubashi, M., Inokuchi, A., & Nishimura, Y. (2002). Head and neck cancer antigens recognized by the humoral immune system. Biochemical and Biophysical Research Communications, 294(3), 734-741. https://doi.org/10.1016/S0006-291X(02)00543-0