Healing of two-wall intra-bony defects treated with a novel EMD-liquid—A pre-clinical study in monkeys

Yoshinori Shirakata, Richard J. Miron, Yukiya Shinohara, Toshiaki Nakamura, Kotaro Sena, Naoto Horai, Dieter D. Bosshardt, Kazuyuki Noguchi, Anton Sculean

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3 Citations (Scopus)


Aim: To investigate the effect of a novel enamel matrix derivative formulation (EMD-liquid or Osteogain) combined with an absorbable collagen sponge (ACS) on periodontal wound healing in intra-bony defects in monkeys. Materials and Methods: Chronic two-wall intra-bony defects were created at the distal aspect of eight teeth in three monkeys (Macaca fascicularis). The 24 defects were randomly assigned to one of the following treatments: (i) open flap debridement (OFD) + ACS alone, (ii) OFD + Emdogain + ACS (Emdogain/ACS), (iii) OFD + Osteogain + ACS (Osteogain/ACS) or (iv) OFD alone. At 4 months, the animals were euthanized for histologic evaluation. Results: Osteogain/ACS resulted in more consistent formation of cementum, periodontal ligament and bone with limited epithelial proliferation compared to OFD alone, Emdogain/ACS and OFD + ACS. Among the four treatment groups, the Osteogain/ACS group demonstrated the highest amount of regenerated tissues. However, complete periodontal regeneration was not observed in any of the defects in the four groups. Conclusions: The present findings indicate that in two-wall intra-bony defects, reconstructive surgery with Osteogain/ACS appears to be a promising novel approach for facilitating periodontal wound healing/regeneration, thus warranting further clinical testing.

Original languageEnglish
Pages (from-to)1264-1273
Number of pages10
JournalJournal of Clinical Periodontology
Issue number12
Publication statusPublished - Dec 2017
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Periodontics


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