Heat-inducible TNF-α gene therapy combined with hyperthermia using magnetic nanoparticles as a novel tumor-targeted therapy

A. Ito, M. Shinkai, H. Honda, T. Kobayashi

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Heat-induced therapeutic gene expression is highly desired for gene therapy to minimize side effects. Furthermore, if the gene expression is triggered by heat stress, combined therapeutic effects of hyperthermia and gene therapy may be possible. We combined TNF-α gene therapy driven by the stress-inducible promoter, gadd 153, with hyperthermia using magnetite cationic liposomes (MCLs). In nude mice, MCLs induced cell death throughout much of the tumor area on heating under an alternating magnetic field. This heat stress also resulted in a 3-fold increase in TNF-α gene expression driven by the gadd 153 promoter as compared with that of nonheated tumor. TNF-α gene expression was also observed in the peripheral area where the hyperthermic effect was not enough to cause cell death. The combined treatment strongly arrested tumor growth in nude mice over a 30-day period, suggesting potential for cancer treatment.

Original languageEnglish
Pages (from-to)649-654
Number of pages6
JournalCancer Gene Therapy
Volume8
Issue number9
DOIs
Publication statusPublished - Oct 10 2001

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Cancer Research

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