Heat shock cognate 70 functions as a chaperone for the stability of kinetochore protein cenp-n in holocentric insect silkworms

Bingqian Li, Zhiqing Li, Chenchen Lu, Li Chang, Dongchao Zhao, Guanwang Shen, Takahiro Kusakabe, Qingyou Xia, Ping Zhao

Research output: Contribution to journalArticle

Abstract

The centromere, in which kinetochore proteins are assembled, plays an important role in the accurate congression and segregation of chromosomes during cell mitosis. Although the function of the centromere and kinetochore is conserved from monocentric to holocentric, the DNA sequences of the centromere and components of the kinetochore are varied among different species. Given the lack of core centromere protein A (CENP-A) and CENP-C in the lepidopteran silkworm Bombyx mori, which possesses holocentric chromosomes, here we investigated the role of CENP-N, another important member of the centromere protein family essential for kinetochore assembly. For the first time, cellular localization and RNA interference against CENP-N have confirmed its kinetochore function in silkworms. To gain further insights into the regulation of CENP-N in the centromere, we analyzed the affinity-purified complex of CENP-N by mass spectrometry and identified 142 interacting proteins. Among these factors, we found that the chaperone protein heat shock cognate 70 (HSC70) is able to regulate the stability of CENP-N by prohibiting ubiquitin–proteasome pathway, indicating that HSC70 could control cell cycle-regulated degradation of CENP-N at centromeres. Altogether, the present work will provide a novel clue to understand the regulatory mechanism for the kinetochore activity of CENP-N during the cell cycle.

Original languageEnglish
Article number5823
JournalInternational journal of molecular sciences
Volume20
Issue number23
DOIs
Publication statusPublished - Dec 1 2019

Fingerprint

silkworms
Kinetochores
Bombyx
insects
Centromere
Protein Stability
Insects
Shock
Hot Temperature
shock
Chromosomes
proteins
Proteins
heat
HSC70 Heat-Shock Proteins
Cells
chromosomes
DNA sequences
Mass spectrometry
mitosis

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Heat shock cognate 70 functions as a chaperone for the stability of kinetochore protein cenp-n in holocentric insect silkworms. / Li, Bingqian; Li, Zhiqing; Lu, Chenchen; Chang, Li; Zhao, Dongchao; Shen, Guanwang; Kusakabe, Takahiro; Xia, Qingyou; Zhao, Ping.

In: International journal of molecular sciences, Vol. 20, No. 23, 5823, 01.12.2019.

Research output: Contribution to journalArticle

Li, Bingqian ; Li, Zhiqing ; Lu, Chenchen ; Chang, Li ; Zhao, Dongchao ; Shen, Guanwang ; Kusakabe, Takahiro ; Xia, Qingyou ; Zhao, Ping. / Heat shock cognate 70 functions as a chaperone for the stability of kinetochore protein cenp-n in holocentric insect silkworms. In: International journal of molecular sciences. 2019 ; Vol. 20, No. 23.
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