Heat shock-inducible expression vectors for use in Schizosaccharomyces pombe

Yasuko Fujita, Hideki Tohda, Yuko Giga-Hama, Kaoru Takegawa

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A new, heat shock-inducible expression system based on an endogenous hsp16+ promoter was developed for use in the fission yeast Schizosaccharomyces pombe. Analysis of GFP expression profiles indicated that a 1.2-kb segment of the hsp16+ promoter region was sufficient to drive expression of heterologous protein. The hsp16+ promoter was found to be activated not only by heat shock but also by other stresses including cadmium, ethanol, and oxidative stress. Two expression vectors, pHIL and pHIU, were constructed using the 1.2-kb hsp16+ promoter for inducible gene expression in Sch. pombe. This new expression system utilizes a simple induction protocol and promises to be a useful tool for analyzing gene expression in Sch. pombe.

Original languageEnglish
Pages (from-to)883-887
Number of pages5
JournalFEMS Yeast Research
Volume6
Issue number6
DOIs
Publication statusPublished - Sep 1 2006
Externally publishedYes

Fingerprint

Schizosaccharomyces
Shock
Hot Temperature
Gene Expression
Cadmium
Genetic Promoter Regions
Oxidative Stress
Ethanol
Proteins

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Infectious Diseases

Cite this

Heat shock-inducible expression vectors for use in Schizosaccharomyces pombe. / Fujita, Yasuko; Tohda, Hideki; Giga-Hama, Yuko; Takegawa, Kaoru.

In: FEMS Yeast Research, Vol. 6, No. 6, 01.09.2006, p. 883-887.

Research output: Contribution to journalArticle

Fujita, Yasuko ; Tohda, Hideki ; Giga-Hama, Yuko ; Takegawa, Kaoru. / Heat shock-inducible expression vectors for use in Schizosaccharomyces pombe. In: FEMS Yeast Research. 2006 ; Vol. 6, No. 6. pp. 883-887.
@article{bc2afee146714e44a26690fa43887edf,
title = "Heat shock-inducible expression vectors for use in Schizosaccharomyces pombe",
abstract = "A new, heat shock-inducible expression system based on an endogenous hsp16+ promoter was developed for use in the fission yeast Schizosaccharomyces pombe. Analysis of GFP expression profiles indicated that a 1.2-kb segment of the hsp16+ promoter region was sufficient to drive expression of heterologous protein. The hsp16+ promoter was found to be activated not only by heat shock but also by other stresses including cadmium, ethanol, and oxidative stress. Two expression vectors, pHIL and pHIU, were constructed using the 1.2-kb hsp16+ promoter for inducible gene expression in Sch. pombe. This new expression system utilizes a simple induction protocol and promises to be a useful tool for analyzing gene expression in Sch. pombe.",
author = "Yasuko Fujita and Hideki Tohda and Yuko Giga-Hama and Kaoru Takegawa",
year = "2006",
month = "9",
day = "1",
doi = "10.1111/j.1567-1364.2006.00093.x",
language = "English",
volume = "6",
pages = "883--887",
journal = "FEMS Yeast Research",
issn = "1567-1356",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Heat shock-inducible expression vectors for use in Schizosaccharomyces pombe

AU - Fujita, Yasuko

AU - Tohda, Hideki

AU - Giga-Hama, Yuko

AU - Takegawa, Kaoru

PY - 2006/9/1

Y1 - 2006/9/1

N2 - A new, heat shock-inducible expression system based on an endogenous hsp16+ promoter was developed for use in the fission yeast Schizosaccharomyces pombe. Analysis of GFP expression profiles indicated that a 1.2-kb segment of the hsp16+ promoter region was sufficient to drive expression of heterologous protein. The hsp16+ promoter was found to be activated not only by heat shock but also by other stresses including cadmium, ethanol, and oxidative stress. Two expression vectors, pHIL and pHIU, were constructed using the 1.2-kb hsp16+ promoter for inducible gene expression in Sch. pombe. This new expression system utilizes a simple induction protocol and promises to be a useful tool for analyzing gene expression in Sch. pombe.

AB - A new, heat shock-inducible expression system based on an endogenous hsp16+ promoter was developed for use in the fission yeast Schizosaccharomyces pombe. Analysis of GFP expression profiles indicated that a 1.2-kb segment of the hsp16+ promoter region was sufficient to drive expression of heterologous protein. The hsp16+ promoter was found to be activated not only by heat shock but also by other stresses including cadmium, ethanol, and oxidative stress. Two expression vectors, pHIL and pHIU, were constructed using the 1.2-kb hsp16+ promoter for inducible gene expression in Sch. pombe. This new expression system utilizes a simple induction protocol and promises to be a useful tool for analyzing gene expression in Sch. pombe.

UR - http://www.scopus.com/inward/record.url?scp=33747331178&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747331178&partnerID=8YFLogxK

U2 - 10.1111/j.1567-1364.2006.00093.x

DO - 10.1111/j.1567-1364.2006.00093.x

M3 - Article

VL - 6

SP - 883

EP - 887

JO - FEMS Yeast Research

JF - FEMS Yeast Research

SN - 1567-1356

IS - 6

ER -