Heightened amygdala long-term potentiation in neurotensin receptor type-1 knockout mice

Taiju Amano, Etsuko Wada, Daisuke Yamada, Ko Zushida, Hiroshi Maeno, Mami Noda, Keiji Wada, Masayuki Sekiguchi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Neurotensin receptor type-1 (Ntsr1) is the main receptor subtype that underlies neurotensin (NT)-mediated modulation of the dopamine (DA) system. Although NT and DA coexist in the basolateral nucleus of the amygdala (BLA), the function of Ntsr1 in the amygdala is not well characterized. In the present study, we utilized Ntsr1 knockout (Ntsr1-KO) mice to examine the role of Ntsr1 in the amygdala. In acute brain slices of Ntsr1-KO mice, synaptic currents elicited in BLA pyramidal neurons by electrical stimulation of the lateral nucleus of the amygdala (LA) were greatly potentiated by tetanic stimulation (BLA-long-term potentiation (LTP)). Such potentiation was not evident in pyramidal neurons of wild-type mice. In the presence of an antagonist of Ntsr1, SR48692, BLA-LTP was consistently observed in the neurons of wild-type mice, suggesting that both inherited deletion and acute pharmacological blockade of Ntsr1 induce BLA-LTP. BLA-LTP in Ntsr1-KO mice was impaired by sulpiride, a DA D2-like receptor antagonist. Conversely, quinpirole, a D 2-like receptor agonist, induced pronounced BLA-LTP in wild-type mice, suggesting the upregulation of D2-like receptor activity in Ntsr1-KO mice. The ratio of NMDA receptor-mediated to non-NMDA receptor-mediated synaptic currents in Ntsr1-KO mouse BLA neurons was approximately double that measured in wild-type mouse neurons. Furthermore, quinpirole potentiated NMDA receptor-mediated synaptic currents in the BLA of wild-type mice. These results suggest that, without Ntsr1, synaptic responses from the LA to BLA pyramidal neurons undergo LTP in response to tetanus stimulation through facilitation of D2-like receptor-induced activation of NMDA receptors.

Original languageEnglish
Pages (from-to)3135-3145
Number of pages11
JournalNeuropsychopharmacology
Volume33
Issue number13
DOIs
Publication statusPublished - Dec 1 2008

Fingerprint

Long-Term Potentiation
Amygdala
Knockout Mice
Pyramidal Cells
N-Methyl-D-Aspartate Receptors
Quinpirole
Neurotensin
Neurons
neurotensin type 1 receptor
Dopamine
Basolateral Nuclear Complex
Sulpiride
Neurotransmitter Receptor
Tetanus
Electric Stimulation
Up-Regulation
Pharmacology

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Psychiatry and Mental health

Cite this

Amano, T., Wada, E., Yamada, D., Zushida, K., Maeno, H., Noda, M., ... Sekiguchi, M. (2008). Heightened amygdala long-term potentiation in neurotensin receptor type-1 knockout mice. Neuropsychopharmacology, 33(13), 3135-3145. https://doi.org/10.1038/npp.2008.38

Heightened amygdala long-term potentiation in neurotensin receptor type-1 knockout mice. / Amano, Taiju; Wada, Etsuko; Yamada, Daisuke; Zushida, Ko; Maeno, Hiroshi; Noda, Mami; Wada, Keiji; Sekiguchi, Masayuki.

In: Neuropsychopharmacology, Vol. 33, No. 13, 01.12.2008, p. 3135-3145.

Research output: Contribution to journalArticle

Amano, T, Wada, E, Yamada, D, Zushida, K, Maeno, H, Noda, M, Wada, K & Sekiguchi, M 2008, 'Heightened amygdala long-term potentiation in neurotensin receptor type-1 knockout mice', Neuropsychopharmacology, vol. 33, no. 13, pp. 3135-3145. https://doi.org/10.1038/npp.2008.38
Amano, Taiju ; Wada, Etsuko ; Yamada, Daisuke ; Zushida, Ko ; Maeno, Hiroshi ; Noda, Mami ; Wada, Keiji ; Sekiguchi, Masayuki. / Heightened amygdala long-term potentiation in neurotensin receptor type-1 knockout mice. In: Neuropsychopharmacology. 2008 ; Vol. 33, No. 13. pp. 3135-3145.
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