TY - JOUR
T1 - Hematopoietic Engraftment in Recipients of Unrelated Donor Umbilical Cord Blood Is Affected by the CD34+ and CD8+ Cell Doses
AU - Terakura, Seitaro
AU - Azuma, Eiichi
AU - Murata, Makoto
AU - Kumamoto, Tadashi
AU - Hirayama, Masahiro
AU - Atsuta, Yoshiko
AU - Kodera, Yoshihisa
AU - Yazaki, Makoto
AU - Naoe, Tomoki
AU - Kato, Koji
N1 - Funding Information:
This work was supported by a Research Grant on Human Genome, Tissue Engineering from the Ministry of Health, Labour and Welfare of Japan (to E.A. and M.M.). We would like to thank the staff of the Japan Cord Blood Network and its attendant transplant centers. We are especially grateful for the help of Drs. Kiyoaki Suzuki, Tadayuki Ishimaru, Kaoru Ishikawa, and Takami Inoue in collecting the UCB, as well as the assistance of Mses. Tomoko Ito, Makiko Shibayama, Hiromi Ogawa, and Nobuko Ishikawa in processing the UCB units.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007/7
Y1 - 2007/7
N2 - Umbilical cord blood (UCB) transplantation is limited by the low number of hematopoietic stem cells in UCB units, which results in a low engraftment rate in transplant recipients. Here, we measured the total nucleated cell count and CD34+, CD3+, CD4+, CD8+, CD14+, and CD16+/56+ cell doses in each UCB unit and evaluated their influence on engraftment and other outcomes in 146 recipients. Multivariate analysis showed a significant association between a higher incidence of successful engraftment and a dose of CD34+ and CD8+ cells above the median (1.4 × 105 and 15.7 × 105 cells/kg, respectively). Engraftment occurred 4 days earlier in patients who received UCB with more than the median dose of CD34+ cells than those receiving UCB at or below the median. Stratification of the group according to CD34+ cell dose revealed a significant influence of the CD8+ cell dose on the time to achieve neutrophil engraftment in patients receiving a lower CD34+ cell dose, whereas there was no significant influence in the patients receiving a higher CD34+ cell dose. These results suggest that consideration of CD34+ and CD8+ cell doses in UCB units may improve the engraftment in recipients of UCB transplantation.
AB - Umbilical cord blood (UCB) transplantation is limited by the low number of hematopoietic stem cells in UCB units, which results in a low engraftment rate in transplant recipients. Here, we measured the total nucleated cell count and CD34+, CD3+, CD4+, CD8+, CD14+, and CD16+/56+ cell doses in each UCB unit and evaluated their influence on engraftment and other outcomes in 146 recipients. Multivariate analysis showed a significant association between a higher incidence of successful engraftment and a dose of CD34+ and CD8+ cells above the median (1.4 × 105 and 15.7 × 105 cells/kg, respectively). Engraftment occurred 4 days earlier in patients who received UCB with more than the median dose of CD34+ cells than those receiving UCB at or below the median. Stratification of the group according to CD34+ cell dose revealed a significant influence of the CD8+ cell dose on the time to achieve neutrophil engraftment in patients receiving a lower CD34+ cell dose, whereas there was no significant influence in the patients receiving a higher CD34+ cell dose. These results suggest that consideration of CD34+ and CD8+ cell doses in UCB units may improve the engraftment in recipients of UCB transplantation.
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U2 - 10.1016/j.bbmt.2007.03.006
DO - 10.1016/j.bbmt.2007.03.006
M3 - Article
C2 - 17580260
AN - SCOPUS:34250196843
VL - 13
SP - 822
EP - 830
JO - Biology of Blood and Marrow Transplantation
JF - Biology of Blood and Marrow Transplantation
SN - 1083-8791
IS - 7
ER -