TY - JOUR
T1 - Heme-undecapeptide labeling on insulin for the immunoassay of insulin with chemiluminescence detection
AU - Zaitsu, Kiyoshi
AU - Kimura, Yoshitada
AU - Ohba, Yoshihito
AU - Hamase, Kenji
AU - Motomura, Yuji
AU - Itose, Minoru
AU - Ishiyama, Masato
PY - 1999/9
Y1 - 1999/9
N2 - Heme-undecapeptide (HUP, microperoxidase-11) labeled insulins, in which the Lys(3) amino group of HUP was crosslinked to one amino group of insulin (Gly(A1), Phe(B1) or Lys(B29)) via a disulfide linkage, were prepared. Lys(3)-[3(2-pyridyldithio) propionoyl]-HUP (Lys(3)-PDP-HUP) was synthesized by the reaction of HUP with N-succinimidyl 3-(2-pyridyldithio)propionate and then conjugated with Gly(A1)-thioglycoloyl- (Gly(A1)-TG-), Phe(B1)-TG- or Lys(B29)TG-insulin. Each TG-insulin was generated by deacetylation of Gly(A1)-acetylthioglycoloyl-(Gly(A1)-ATG-), Phe(B1)-ATG- or Lys(B29)-ATG- insulin, that was prepared by reacting native insulin, Phe(B1)-3,4,5,6- tetrahydrophthalyl-insulin (Phe(B1)-THP-insulin) or Gly(A1),Phe(B1)-(THP)2- insulin with N-succinimidyl S-acetylthioacetate (SATA), respectively, followed by deprotection of THP, a reversible amino-protecting group. Both preparative reversed-phase and anion-exchange HPLC were used in the syntheses of PDP-HUP and the insulin derivatives (THP-, ATG- and HUP-insulin), respectively. The optimal reaction conditions for these syntheses were extensively studied using HPLC separation. In addition, immunoreactivity of Phe(B1)-HUP-insulin was manifested by using a solid-phase antibody method with chemiluminescence detection.
AB - Heme-undecapeptide (HUP, microperoxidase-11) labeled insulins, in which the Lys(3) amino group of HUP was crosslinked to one amino group of insulin (Gly(A1), Phe(B1) or Lys(B29)) via a disulfide linkage, were prepared. Lys(3)-[3(2-pyridyldithio) propionoyl]-HUP (Lys(3)-PDP-HUP) was synthesized by the reaction of HUP with N-succinimidyl 3-(2-pyridyldithio)propionate and then conjugated with Gly(A1)-thioglycoloyl- (Gly(A1)-TG-), Phe(B1)-TG- or Lys(B29)TG-insulin. Each TG-insulin was generated by deacetylation of Gly(A1)-acetylthioglycoloyl-(Gly(A1)-ATG-), Phe(B1)-ATG- or Lys(B29)-ATG- insulin, that was prepared by reacting native insulin, Phe(B1)-3,4,5,6- tetrahydrophthalyl-insulin (Phe(B1)-THP-insulin) or Gly(A1),Phe(B1)-(THP)2- insulin with N-succinimidyl S-acetylthioacetate (SATA), respectively, followed by deprotection of THP, a reversible amino-protecting group. Both preparative reversed-phase and anion-exchange HPLC were used in the syntheses of PDP-HUP and the insulin derivatives (THP-, ATG- and HUP-insulin), respectively. The optimal reaction conditions for these syntheses were extensively studied using HPLC separation. In addition, immunoreactivity of Phe(B1)-HUP-insulin was manifested by using a solid-phase antibody method with chemiluminescence detection.
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U2 - 10.2116/analsci.15.871
DO - 10.2116/analsci.15.871
M3 - Article
AN - SCOPUS:0033383821
SN - 0910-6340
VL - 15
SP - 871
EP - 878
JO - Analytical Sciences
JF - Analytical Sciences
IS - 9
ER -