Heparin-conjugated gelatin as a growth factor immobilization scaffold

Shintaro Nakamura, Takafumi Kubo, Hiroyuki Ijima

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Tissue engineering requires growth factors, cells and a scaffold to permit effective tissue regeneration. This study aimed to develop a scaffold with a focus on immobilizing growth factors within gelatin. We focused on the extracellular matrix and developed a heparin-conjugated gelatin (Hep-gela). Conjugation was confirmed using the alcian blue assay and X-ray diffraction patterns. The mechanical strength and stability of the Hep-gela gel in protease solution were improved compared with collagen gel. Hep-gela was able to immobilize vascular endothelial growth factor (VEGF) even in the presence of albumin, with an efficiency of 54.2%. Immobilized VEGF promoted proliferation of human umbilical vein endothelial cells. Hep-gela-immobilized VEGF maintained its native biological activity. In summary, Hep-gela has the potential to become an effective material in the field of regenerative medicine.

Original languageEnglish
Pages (from-to)562-567
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume115
Issue number5
DOIs
Publication statusPublished - May 1 2013

Fingerprint

Gelatin
Scaffolds (biology)
Immobilization
Heparin
Intercellular Signaling Peptides and Proteins
Vascular Endothelial Growth Factor A
Scaffolds
Gels
Tissue regeneration
Mechanical stability
Endothelial cells
Alcian Blue
Bioactivity
Regenerative Medicine
Tissue engineering
Collagen
Diffraction patterns
Human Umbilical Vein Endothelial Cells
Strength of materials
Tissue Engineering

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Heparin-conjugated gelatin as a growth factor immobilization scaffold. / Nakamura, Shintaro; Kubo, Takafumi; Ijima, Hiroyuki.

In: Journal of Bioscience and Bioengineering, Vol. 115, No. 5, 01.05.2013, p. 562-567.

Research output: Contribution to journalArticle

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