TY - JOUR
T1 - Heparin-conjugated gelatin as a growth factor immobilization scaffold
AU - Nakamura, Shintaro
AU - Kubo, Takafumi
AU - Ijima, Hiroyuki
N1 - Funding Information:
This work was supported by the Grant-in-Aid for Scientific Research (B): 22360348 from the Ministry of Education, Culture, Sports, Science and Technology of Japan . The analysis X-ray Diffraction of Hep-gela was performed using MultiFlex at the Center of Advanced Instrumental Analysis, Kyushu University.
PY - 2013/5
Y1 - 2013/5
N2 - Tissue engineering requires growth factors, cells and a scaffold to permit effective tissue regeneration. This study aimed to develop a scaffold with a focus on immobilizing growth factors within gelatin. We focused on the extracellular matrix and developed a heparin-conjugated gelatin (Hep-gela). Conjugation was confirmed using the alcian blue assay and X-ray diffraction patterns. The mechanical strength and stability of the Hep-gela gel in protease solution were improved compared with collagen gel. Hep-gela was able to immobilize vascular endothelial growth factor (VEGF) even in the presence of albumin, with an efficiency of 54.2%. Immobilized VEGF promoted proliferation of human umbilical vein endothelial cells. Hep-gela-immobilized VEGF maintained its native biological activity. In summary, Hep-gela has the potential to become an effective material in the field of regenerative medicine.
AB - Tissue engineering requires growth factors, cells and a scaffold to permit effective tissue regeneration. This study aimed to develop a scaffold with a focus on immobilizing growth factors within gelatin. We focused on the extracellular matrix and developed a heparin-conjugated gelatin (Hep-gela). Conjugation was confirmed using the alcian blue assay and X-ray diffraction patterns. The mechanical strength and stability of the Hep-gela gel in protease solution were improved compared with collagen gel. Hep-gela was able to immobilize vascular endothelial growth factor (VEGF) even in the presence of albumin, with an efficiency of 54.2%. Immobilized VEGF promoted proliferation of human umbilical vein endothelial cells. Hep-gela-immobilized VEGF maintained its native biological activity. In summary, Hep-gela has the potential to become an effective material in the field of regenerative medicine.
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U2 - 10.1016/j.jbiosc.2012.11.011
DO - 10.1016/j.jbiosc.2012.11.011
M3 - Article
C2 - 23273911
AN - SCOPUS:84876306845
VL - 115
SP - 562
EP - 567
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 5
ER -
