TY - JOUR
T1 - Hepatic cholesterol 7α-hydroxylase activity and serum 7α-hydroxy- cholesterol level during liver regeneration after partial hepatectomy in rats
AU - Nakano, K.
AU - Chijiiwa, K.
AU - Okamoto, S.
AU - Yamashita, H.
AU - Kuroki, S.
AU - Tanaka, M.
PY - 1995
Y1 - 1995
N2 - Hepatic cholesterol 7α -hydroxylase is a rate-limiting enzyme for bile acid synthesis, and we have previously shown that the serum 7α-hydroxycholesterol level reflects the enzyme activity in patients with an intact liver. The purpose of this study was to evaluate the alterations in hepatic cholesterol 7α-hydroxy- lase activity and the serum 7α-hydroxycholesterol level after hepatectomy and the correlations between these parameters and liver mass. Hepatic cholesterol 7α-hydroxylase activity, the serum 7α-hydroxycholesterol level and the remaining liver weight were determined after 70% hepatectomy in rats. Cholesterol 7α-hydroxylase activity was decreased on days 1 and 2 (from 28.3 ± 3.7 to 8.5 ± 2.8 and 13.3 ± 3.6 pmol/min/mg protein, respectively), returned to the preoperative level on day 3 (24.7 ± 2.6 pmol/min/mg protein) and further elevated thereafter (71.4 ± 10.9 on day 7 and 51.3 ± 6.6 pmol/min/mg protein on day 14). The serum level of 7α-hydroxycholesterol changed simultaneously with the enzyme activity, and a significant correlation between the two parameters was observed (r = 0.915, p < 0.0001). The correlations between the liver weight and hepatic cholesterol 7α-hydroxylase activity and serum 7α-hydroxycholesterol level were significant (p < 0.01), but the significances disappeared for the period of days 3-14 (r = 0.424, p = 0.08; r = 0.299, p = 0.228, respectively). In conclusion, hepatic cholesterol 7α-hydroxylase activity is sup-pressed by hepatectomy and then activated as the liver regenerates. The serum 7α-hydroxycholesterol level is a good parameter of this enzyme activity after partial hepatectomy.
AB - Hepatic cholesterol 7α -hydroxylase is a rate-limiting enzyme for bile acid synthesis, and we have previously shown that the serum 7α-hydroxycholesterol level reflects the enzyme activity in patients with an intact liver. The purpose of this study was to evaluate the alterations in hepatic cholesterol 7α-hydroxy- lase activity and the serum 7α-hydroxycholesterol level after hepatectomy and the correlations between these parameters and liver mass. Hepatic cholesterol 7α-hydroxylase activity, the serum 7α-hydroxycholesterol level and the remaining liver weight were determined after 70% hepatectomy in rats. Cholesterol 7α-hydroxylase activity was decreased on days 1 and 2 (from 28.3 ± 3.7 to 8.5 ± 2.8 and 13.3 ± 3.6 pmol/min/mg protein, respectively), returned to the preoperative level on day 3 (24.7 ± 2.6 pmol/min/mg protein) and further elevated thereafter (71.4 ± 10.9 on day 7 and 51.3 ± 6.6 pmol/min/mg protein on day 14). The serum level of 7α-hydroxycholesterol changed simultaneously with the enzyme activity, and a significant correlation between the two parameters was observed (r = 0.915, p < 0.0001). The correlations between the liver weight and hepatic cholesterol 7α-hydroxylase activity and serum 7α-hydroxycholesterol level were significant (p < 0.01), but the significances disappeared for the period of days 3-14 (r = 0.424, p = 0.08; r = 0.299, p = 0.228, respectively). In conclusion, hepatic cholesterol 7α-hydroxylase activity is sup-pressed by hepatectomy and then activated as the liver regenerates. The serum 7α-hydroxycholesterol level is a good parameter of this enzyme activity after partial hepatectomy.
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U2 - 10.1159/000129425
DO - 10.1159/000129425
M3 - Article
C2 - 8542924
AN - SCOPUS:0028875871
VL - 27
SP - 389
EP - 395
JO - European Surgical Research
JF - European Surgical Research
SN - 0014-312X
IS - 6
ER -