TY - JOUR
T1 - Hepatoblasts comprise a niche for fetal liver erythropoiesis through cytokine production
AU - Sugiyama, Daisuke
AU - Kulkeaw, Kasem
AU - Mizuochi, Chiyo
AU - Horio, Yuka
AU - Okayama, Satoko
N1 - Funding Information:
The authors acknowledge grant support from the Special Coordination Funds for Promoting Science and Technology , a Grant-in-Aid for Young Scientists (B) from The Ministry of Education, Culture, Sports, Science and Technology , Grant-in-Aid by Ministry of Health, Labor and Welfare, Bilateral Joint Projects for Japan Society for the Promotion of Science, the Ajinomoto Scholarship Foundation and the Tokyo Biochemical Research Foundation. We thank the Research Support Center, the Graduate School of Medical Sciences, Kyushu University, for technical support, Drs. K. Akashi and K. Tani for helpful discussion, Dr. T. Inoue for technical support, and Dr. Elise Lamar for critical reading of the manuscript.
PY - 2011/7/1
Y1 - 2011/7/1
N2 - In mammals, definitive erythropoiesis first occurs in fetal liver (FL), although little is known about how the process is regulated. FL consists of hepatoblasts, sinusoid endothelial cells and hematopoietic cells. To determine niche cells for fetal liver erythropoiesis, we isolated each FL component by flow cytometry. mRNA analysis suggested that Dlk-1-expressing hepatoblasts primarily expressed EPO and SCF, genes encoding erythropoietic cytokines. EPO protein was detected predominantly in hepatoblasts, as assessed by ELISA and immunohistochemistry, and was not detected in sinusoid endothelial cells and hematopoietic cells. To characterize hepatoblast function in FL, we analyzed Map2k4-/- mouse embryos, which lack hepatoblasts, and observed down-regulation of EPO and SCF expression in FL relative to wild-type mice. Our observations demonstrate that hepatoblasts comprise a niche for erythropoiesis through cytokine secretion.
AB - In mammals, definitive erythropoiesis first occurs in fetal liver (FL), although little is known about how the process is regulated. FL consists of hepatoblasts, sinusoid endothelial cells and hematopoietic cells. To determine niche cells for fetal liver erythropoiesis, we isolated each FL component by flow cytometry. mRNA analysis suggested that Dlk-1-expressing hepatoblasts primarily expressed EPO and SCF, genes encoding erythropoietic cytokines. EPO protein was detected predominantly in hepatoblasts, as assessed by ELISA and immunohistochemistry, and was not detected in sinusoid endothelial cells and hematopoietic cells. To characterize hepatoblast function in FL, we analyzed Map2k4-/- mouse embryos, which lack hepatoblasts, and observed down-regulation of EPO and SCF expression in FL relative to wild-type mice. Our observations demonstrate that hepatoblasts comprise a niche for erythropoiesis through cytokine secretion.
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U2 - 10.1016/j.bbrc.2011.05.137
DO - 10.1016/j.bbrc.2011.05.137
M3 - Article
C2 - 21664343
AN - SCOPUS:79959715298
SN - 0006-291X
VL - 410
SP - 301
EP - 306
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -