Hepatoblasts comprise a niche for fetal liver erythropoiesis through cytokine production

Daisuke Sugiyama, Kasem Kulkeaw, Chiyo Mizuochi, Yuka Horio, Satoko Okayama

    Research output: Contribution to journalArticlepeer-review

    26 Citations (Scopus)

    Abstract

    In mammals, definitive erythropoiesis first occurs in fetal liver (FL), although little is known about how the process is regulated. FL consists of hepatoblasts, sinusoid endothelial cells and hematopoietic cells. To determine niche cells for fetal liver erythropoiesis, we isolated each FL component by flow cytometry. mRNA analysis suggested that Dlk-1-expressing hepatoblasts primarily expressed EPO and SCF, genes encoding erythropoietic cytokines. EPO protein was detected predominantly in hepatoblasts, as assessed by ELISA and immunohistochemistry, and was not detected in sinusoid endothelial cells and hematopoietic cells. To characterize hepatoblast function in FL, we analyzed Map2k4-/- mouse embryos, which lack hepatoblasts, and observed down-regulation of EPO and SCF expression in FL relative to wild-type mice. Our observations demonstrate that hepatoblasts comprise a niche for erythropoiesis through cytokine secretion.

    Original languageEnglish
    Pages (from-to)301-306
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume410
    Issue number2
    DOIs
    Publication statusPublished - Jul 1 2011

    All Science Journal Classification (ASJC) codes

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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