Hepatocyte growth factor and invasion-stimulatory activity are induced in pleural fluid by surgery in lung cancer patients

A. Uchiyama, T. Morlsaki, K. Beppu, M. Kojima, Y. Matsunari, A. Nakatsuka, K. Mizumoto, K. Matsumoto, T. Nakamura, M. Tanaka

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Hepatocyte growth factor (HGF) is a stromal cell-derived cytokine that can stimulate matrix invasion by carcinoma cells. We analysed the concentrations of HGF and invasion-stimulatory activity in pleural fluid after lung surgery. The concentration of HGF in pleural fluids was measured by enzyme-linked immunosorbent assay in seven patients who underwent pulmonary resection for primary or metastatic lung cancer. The effect of the pleural fluid on cancer cell invasion across reconstituted basement membrane (Matrigel) was assessed with a Boyden chamber assay using a lung adenocarcinoma cell line, A549. HGF levels in the pleural fluid alter lung surgery ranged from 6.0 to 23.0 ng ml-1 (average: 10.2 ± 4.3 ng ml-1). The matrix invasion of lung carcinoma cells in the presence of the pleural fluid was significantly higher than that in the presence of culture medium alone or sera from normal subjects (P < 0.01). The invasion-stimulatory activity of the pleural fluid was strongly inhibited by HGF-neutralizing antibody. Positive correlation was found between the HGF level and invasion-stimulatory activity in the pleural fluids and normal sera (P = 0.0073). This is the first report demonstrating that the lung surgery induces a considerable amount of HGF, which is closely correlated with the invasion-stimulatory activity of the pleural fluid.

Original languageEnglish
Pages (from-to)721-726
Number of pages6
JournalBritish journal of cancer
Volume81
Issue number4
DOIs
Publication statusPublished - Oct 11 1999

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Uchiyama, A., Morlsaki, T., Beppu, K., Kojima, M., Matsunari, Y., Nakatsuka, A., ... Tanaka, M. (1999). Hepatocyte growth factor and invasion-stimulatory activity are induced in pleural fluid by surgery in lung cancer patients. British journal of cancer, 81(4), 721-726. https://doi.org/10.1038/sj.bjc.6690754