Hepatoprotective activities of the methanol extract of Angelica shikokiana and isoepoxypteryxin against hepatocellular carcinoma

A. Mira, N. Elsherbiny, W. Alkhiary, A. Shebl, H. Tran, Kuniyoshi Shimizu

Research output: Contribution to journalArticle

Abstract

Angelica shikokiana (Apiaceae) is a Japanese traditional herb and widely marketed as a dietary food supplement as a health tea preparation consumed as a daily beverage for its health benefits. Our previous research showed that the methanol extract of the aerial parts of A. shikokiana and its major coumarin, isoepoxypteryxin had a significant cytotoxicity against HepG2 cell line. In this study, we investigated the hepatoprotective activity more deeply by the in vitro inhibition of HepG2 cells invasion assay and in vivo using thioacetamide-induced hepatocellular carcinoma model in rats. The ex vivo levels of nitric oxide production, inducible nitric oxide synthase, vascular endothelial cell growth factor-C and caspase-3 were also estimated to investigate the possible mechanisms of hepatoprotection. The methanol extract of A. shikokiana and isoepoxypteryxin concentration-dependently depressed the invasion of HepG2 cells induced by 12-O-tetradecanoylphorbol 13-acetate. In vivo, they significantly reduced the levels of alanine transaminase, aspartate transaminase, gamma-glutamyl transpeptidase, total bilirubin and nitric oxide production when compared with hepatocellular carcinoma group. Besides, the thioacetamide-injected groups treated with methanol extract of A. shikokiana and isoepoxypteryxin exhibited a significant increase in the level of caspase-3 protein and a significant decrease of inducible nitric oxide synthase and vascular endothelial cell growth factor C levels when compared with hepatocellular carcinoma group. To the best of our knowledge, this is the first report of the hepatoprotective effect of A. shikokiana and isoepoxypteryxin against hepatocellular carcinoma.

Original languageEnglish
Pages (from-to)576-584
Number of pages9
JournalIndian Journal of Pharmaceutical Sciences
Volume79
Issue number4
DOIs
Publication statusPublished - Jan 1 2017

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Angelica
Methanol
Hepatocellular Carcinoma
Hep G2 Cells
Thioacetamide
Vascular Endothelial Growth Factor C
Nitric Oxide Synthase Type II
Dietary Supplements
Caspase 3
Nitric Oxide
Endothelial Cells
Apiaceae
gamma-Glutamyltransferase
Beverages
Tetradecanoylphorbol Acetate
Insurance Benefits
Tea
Aspartate Aminotransferases
Alanine Transaminase
Bilirubin

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

Hepatoprotective activities of the methanol extract of Angelica shikokiana and isoepoxypteryxin against hepatocellular carcinoma. / Mira, A.; Elsherbiny, N.; Alkhiary, W.; Shebl, A.; Tran, H.; Shimizu, Kuniyoshi.

In: Indian Journal of Pharmaceutical Sciences, Vol. 79, No. 4, 01.01.2017, p. 576-584.

Research output: Contribution to journalArticle

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