Hetero-oligomerization of C2 domains of phospholipase C-related but catalytically inactive protein and synaptotagmin-1

Da Guang Wang, Hiroshi Takeuchi, Jing Gao, Zhao Zhang, Masato Hirata

Research output: Contribution to journalArticle

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Abstract

The C2 domain is a protein module often found in molecules that regulate exocytosis. C2 domains mediate interactions between the parental molecule and Ca2+, phospholipids, and proteins. Although various molecules have been shown to interact with several C2 domains, no interactions between the C2 domains from different molecules have yet been reported. In the present study, we identified direct interactions between the C2 domain of PRIP (phospholipase C-related but catalytically inactive protein) and the C2 domains of other molecules. Among the C2 domains examined, those of synaptotagmin-1 (Syt1-C2A and Syt1-C2B) and phospholipase C δ-1 bound to the C2 domain of PRIP. We investigated the interactions between the C2 domain of PRIP (PRIP-C2) with Syt1-C2A and Syt1-C2B, and the mode of binding of each was Ca2+-dependent and -independent, respectively. We further demonstrated that the Ca2+ dependence of the interaction between PRIP-C2 and Syt1-C2A was attributed to Ca2+ binding with Syt1-C2A, but not PRIP-C2, using a series of mutants prepared from both C2 domains. We previously reported that the interaction between PRIP-C2 and the membrane fusion machinery suggested a critical role for PRIP in exocytosis; therefore, the results of the present study further support the importance of PRIP-C2 in the inhibitory function of PRIP in regulating exocytosis.

Original languageEnglish
Pages (from-to)120-129
Number of pages10
JournalAdvances in Biological Regulation
Volume57
DOIs
Publication statusPublished - Jan 1 2015

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Synaptotagmin I
Type C Phospholipases
Proteins
Exocytosis
C2 Domains
Membrane Fusion
Phospholipids

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Hetero-oligomerization of C2 domains of phospholipase C-related but catalytically inactive protein and synaptotagmin-1. / Wang, Da Guang; Takeuchi, Hiroshi; Gao, Jing; Zhang, Zhao; Hirata, Masato.

In: Advances in Biological Regulation, Vol. 57, 01.01.2015, p. 120-129.

Research output: Contribution to journalArticle

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