Heterodimerization of Group I Ribozymes Enabling Exon Recombination through Pairs of Cooperative trans-Splicing Reactions

Takahiro Tanaka, Yusuke Hirata, Yuto Tominaga, Hiroyuki Furuta, Shigeyoshi Matsumura, Yoshiya Ikawa

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    Group I (GI) self-splicing ribozymes are attractive tools for biotechnology and synthetic biology. Several trans-splicing and related reactions based on GI ribozymes have been developed for the purpose of recombining their target mRNA sequences. By combining trans-splicing systems with rational modular engineering of GI ribozymes it was possible to achieve more complex editing of target RNA sequences. In this study we have developed a cooperative trans-splicing system through rational modular engineering with use of dimeric GI ribozymes derived from the Tetrahymena group I intron ribozyme. The resulting pairs of ribozymes exhibited catalytic activity depending on their selective dimerization. Rational modular redesign as performed in this study would facilitate the development of sophisticated regulation of double or multiple trans-splicing reactions in a cooperative manner.

    Original languageEnglish
    Pages (from-to)1659-1667
    Number of pages9
    JournalChemBioChem
    Volume18
    Issue number16
    DOIs
    Publication statusPublished - Aug 17 2017

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Molecular Medicine
    • Molecular Biology
    • Organic Chemistry

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