Heterogeneity in the expression pattern of two ganglioside synthase genes during mouse brain development

Akihito Yamamoto, Masashi Haraguchi, Shuji Yamashiro, Satoshi Fukumoto, Keiko Furukawa, Kogo Takamiya, Mitsuru Atsuta, Hiroshi Shiku, Koichi Furukawa

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Gangliosides are synthesized by sequential catalytic reaction of multiple glycosyltransferases. GM2/GD2 synthase and GD3 synthase are key enzymes for ganglioside synthesis, because their relative activities regulate the main profiles of ganglioside expression. Mouse GD3 synthase (EC 2.4.99.8) cDNA was cloned by eukaryotic expression cloning, and its mRNA expression as well as that of GM2/GD2 synthase gene during the development of the mouse CNS was analyzed by using northern blotting, reverse transcription-polymerase chain reaction, and in situ hybridization. When brain tissue was analyzed as a whole mass, a typical pattern corresponding to the reported findings obtained by biochemical analyses was observed, i.e., high expression of GD3 synthase gene in the early stage and gradual increase of GM2/GD2 synthase gene expression in the late stage of the development. However, the results of in situ hybridization of these two genes revealed that the expression kinetics of these two genes were heterogeneous among various sites in the brain under development. These findings suggest that various expression patterns of the two genes reflect differences in the course of the development of individual sites, and also different ganglioside components are required in individual portions of the brain for development and maintenance of the function.

Original languageEnglish
Pages (from-to)26-34
Number of pages9
JournalJournal of Neurochemistry
Volume66
Issue number1
Publication statusPublished - Jan 1 1996
Externally publishedYes

Fingerprint

(N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase
Gangliosides
Brain
Genes
In Situ Hybridization
Glycosyltransferases
Cloning
Polymerase chain reaction
Gene Expression
Transcription
Gene expression
Northern Blotting
Complementary DNA
Reverse Transcription
Organism Cloning
Tissue
Messenger RNA
Maintenance
Kinetics
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Yamamoto, A., Haraguchi, M., Yamashiro, S., Fukumoto, S., Furukawa, K., Takamiya, K., ... Furukawa, K. (1996). Heterogeneity in the expression pattern of two ganglioside synthase genes during mouse brain development. Journal of Neurochemistry, 66(1), 26-34.

Heterogeneity in the expression pattern of two ganglioside synthase genes during mouse brain development. / Yamamoto, Akihito; Haraguchi, Masashi; Yamashiro, Shuji; Fukumoto, Satoshi; Furukawa, Keiko; Takamiya, Kogo; Atsuta, Mitsuru; Shiku, Hiroshi; Furukawa, Koichi.

In: Journal of Neurochemistry, Vol. 66, No. 1, 01.01.1996, p. 26-34.

Research output: Contribution to journalArticle

Yamamoto, A, Haraguchi, M, Yamashiro, S, Fukumoto, S, Furukawa, K, Takamiya, K, Atsuta, M, Shiku, H & Furukawa, K 1996, 'Heterogeneity in the expression pattern of two ganglioside synthase genes during mouse brain development', Journal of Neurochemistry, vol. 66, no. 1, pp. 26-34.
Yamamoto A, Haraguchi M, Yamashiro S, Fukumoto S, Furukawa K, Takamiya K et al. Heterogeneity in the expression pattern of two ganglioside synthase genes during mouse brain development. Journal of Neurochemistry. 1996 Jan 1;66(1):26-34.
Yamamoto, Akihito ; Haraguchi, Masashi ; Yamashiro, Shuji ; Fukumoto, Satoshi ; Furukawa, Keiko ; Takamiya, Kogo ; Atsuta, Mitsuru ; Shiku, Hiroshi ; Furukawa, Koichi. / Heterogeneity in the expression pattern of two ganglioside synthase genes during mouse brain development. In: Journal of Neurochemistry. 1996 ; Vol. 66, No. 1. pp. 26-34.
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