Heterogeneous induction of apoptosis in colon cancer cells by wild-type p53 gene transfection

Masaaki Namoto, Yoshikazu Yonemitsu, Kazunori Nakagawa, Shuichi Hashimoto, Yasufumi Kaneda, Hajime Nawata, Katsuo Sueishi

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8 Citations (Scopus)

Abstract

To examine the effects of wild-type (wt)-p53 gene transfer on cancer cell growth and apoptosis induction, we transduced human wt-p53 cDNA into three colon cancer cell lines either with or without a mutation of the p53 gene using the HVJ-cationic liposome method. Wt-p53 gene transfer, thus, induced an apparent growth arrest in all cell lines, but itsenhancement of the apoptotic rate varied (from about 4 to 70 times). The simultaneous doxorubicin treatment was able to enhance growth arrest and the apoptosis induction rate. These findings suggest that wt-p53 gene transfer using HVJ- cationic liposomes seems to be a potentially effective therapeutic strategy, however wt-p53 gene transfer still appears to be more effective in combination with other cytotoxic treatments.

Original languageEnglish
Pages (from-to)777-784
Number of pages8
JournalInternational journal of oncology
Volume12
Issue number4
Publication statusPublished - Apr 1 1998

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All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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