Heterogeneous reorganization of actin filaments in living endothelial cellin response to shear stress

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Abstract

In this study, we investigated the spatial and temporal reorganization of actin filaments in living endothelial cells in response to shear stress by transfecting a fluorescent protein, Dronpa-Green-labeled actin, which was photoactivated microscopically by UV irradiation, and evaluating the time constants of fluorescence decay after photoactivation. The time constant in the upstream region decreased gradually after 30 min of shear stress and then increased. Particularly, the time constant in the downstream region tended to be higher than in the upstream region, suggesting that actin polymerization was more activated in the downstream region. Our results demonstrated the spatial and temporal heterogeneity of actin reorganization due to shear stress.

Original languageEnglish
Pages (from-to)18-24
Number of pages7
JournalJournal of Biorheology
Volume34
Issue number1
DOIs
Publication statusPublished - 2020

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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