TY - JOUR
T1 - Heterogony in cycloneuroterus (Hymenoptera
T2 - Cynipidae: Cynipini) from rearing experiments and DNA Barcoding
AU - Ide, Tatsuya
AU - Abe, Yoshihisa
N1 - Funding Information:
We thank K. Matsunaga for providing useful specimens and biological information about the asexual generation of C. gilvus. Information from A. Nagai and T. Komoda prompted us to pay attention to galls on Q. gilva. N. Kanzaki, A. Koyama, S. E. Tanaka, and T. Ekino assisted with the field collections. This study was partly supported by Japan Society for the Promotion of Science grant numbers JP 12J05764 and JP17H07387.
Publisher Copyright:
© 2019 The Author(s) 2019. Published by Oxford University Press on behalf of Entomological Society of America.
PY - 2019/9/19
Y1 - 2019/9/19
N2 - Heterogony was confirmed in the cynipid genus Cycloneuroterus Melika and Tang in rearing experiments with DNA barcoding. These experiments involved Cycloneuroterus gilvus Tang and Melika, which was previously only described from the sexual generation adult. The first rearing experiment was conducted using unidentified asexual generation females collected from Quercus gilva Blume, and gall formation by the sexual generation offspring was confirmed on folded or unfolded young leaves of Q. gilva. The second experiment was conducted using sexual generation males and females reared from the leaf galls collected from Q. gilva, and gall formation by the asexual generation offspring was observed on leaves of Q. gilva. Based on the morphological features of the sexual generation adults and galls, this species was identified as C. gilvus. The species identity of wasp specimens of sexual and asexual generations used in the rearing experiments was cross-checked using DNA barcoding with the partial sequences of the cytochrome c oxidase subunit I (COI) region (658 bp). The asexual generation adult and gall of C. gilvus are described based on these results. The importance of 'closing the life cycle,' in this case a demonstration of heterogony, in oak gall wasps (Cynipini) is discussed.
AB - Heterogony was confirmed in the cynipid genus Cycloneuroterus Melika and Tang in rearing experiments with DNA barcoding. These experiments involved Cycloneuroterus gilvus Tang and Melika, which was previously only described from the sexual generation adult. The first rearing experiment was conducted using unidentified asexual generation females collected from Quercus gilva Blume, and gall formation by the sexual generation offspring was confirmed on folded or unfolded young leaves of Q. gilva. The second experiment was conducted using sexual generation males and females reared from the leaf galls collected from Q. gilva, and gall formation by the asexual generation offspring was observed on leaves of Q. gilva. Based on the morphological features of the sexual generation adults and galls, this species was identified as C. gilvus. The species identity of wasp specimens of sexual and asexual generations used in the rearing experiments was cross-checked using DNA barcoding with the partial sequences of the cytochrome c oxidase subunit I (COI) region (658 bp). The asexual generation adult and gall of C. gilvus are described based on these results. The importance of 'closing the life cycle,' in this case a demonstration of heterogony, in oak gall wasps (Cynipini) is discussed.
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U2 - 10.1093/aesa/saz035
DO - 10.1093/aesa/saz035
M3 - Article
AN - SCOPUS:85073434766
VL - 112
SP - 482
EP - 489
JO - Annals of the Entomological Society of America
JF - Annals of the Entomological Society of America
SN - 0013-8746
IS - 5
ER -